The clonal proliferation in vitro of enriched populations of human promyelocytes and myelocytes.

The clonal proliferation in vitro of enriched populations of human promyelocytes and myelocytes.
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人类早幼粒细胞和骨髓细胞富集群体的体外克隆增殖。

DOI:
10.1182/blood.v65.4.951.951
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发表时间:
1985
期刊:
影响因子:
20.3
通讯作者:
D. Metcalf
D. Metcalf
中科院分区:
医学1区
文献类型:
--
作者:
C. Begley;A. Lopez;M. Vadas;D. Metcalf

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正常人早幼粒细胞和中幼粒细胞的增殖能力是在琼脂克隆增殖的基础上证明和表征的。使用单克隆抗体韦姆G11和荧光激活细胞分选仪(FACS)从骨髓中获得正常人早幼粒细胞和髓细胞的富集群体。在胎盘条件培养基刺激的培养物中,这些细胞在培养的第3天和第5天之间产生峰值总克隆数。随后,克隆人迅速消失。这些克隆在第7天主要为亚菌落大小,尽管该群体产生了一些菌落。这些克隆的类型主要是嗜酸性的。这些细胞的平板接种效率高达50%,克隆增殖依赖于集落刺激因子(CSF)的刺激。
The proliferative capacity of normal human promyelocytes and myelocytes was demonstrated and characterized on the basis of clonal proliferation in agar. An enriched population of normal human promyelocytes and myelocytes was obtained from bone marrow using the monoclonal antibody WEM G11 and the fluorescence-activated cell sorter (FACS). In cultures stimulated by placental-conditioned medium, these cells generated peak total clone numbers between days 3 and 5 of culture. Clones disappeared rapidly thereafter. These clones were mainly of subcolony size at day 7, although some colonies were generated by this population. The clones were primarily neutrophilic in type. These cells had a plating efficiency of up to 50%, and clonal proliferation was dependent on stimulation by colony-stimulating factor (CSF).
DOI: --
发表时间: 1983
期刊: Blood
影响因子: 20.3
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