The clonal proliferation in vitro of enriched populations of human promyelocytes and myelocytes.
The clonal proliferation in vitro of enriched populations of human promyelocytes and myelocytes.
复制标题
人类早幼粒细胞和骨髓细胞富集群体的体外克隆增殖。
DOI:
10.1182/blood.v65.4.951.951
复制
发表时间:
1985
期刊:
影响因子:
20.3
通讯作者:
D. Metcalf
中科院分区:
文献类型:
--
作者:
C. Begley;A. Lopez;M. Vadas;D. Metcalf
The proliferative capacity of normal human promyelocytes and myelocytes was demonstrated and characterized on the basis of clonal proliferation in agar. An enriched population of normal human promyelocytes and myelocytes was obtained from bone marrow using the monoclonal antibody WEM G11 and the fluorescence-activated cell sorter (FACS). In cultures stimulated by placental-conditioned medium, these cells generated peak total clone numbers between days 3 and 5 of culture. Clones disappeared rapidly thereafter. These clones were mainly of subcolony size at day 7, although some colonies were generated by this population. The clones were primarily neutrophilic in type. These cells had a plating efficiency of up to 50%, and clonal proliferation was dependent on stimulation by colony-stimulating factor (CSF).
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影响因子:
20.3
作者:
Strauss,LC;Stuart,RK;Civin,CI
通讯作者:
Civin,CI
影响因子:
20.3
作者:
Ball,ED;Fanger,MW
通讯作者:
Fanger,MW
影响因子:
20.3
作者:
Nicola,NA;Metcalf,D;vonMelchner,H;Burgess,AW
通讯作者:
Burgess,AW
DOI:
--
发表时间:
1980
期刊:
Blood cells
影响因子:
--
作者:
Nicola,NA;Morstyn,G;Metcalf,D
通讯作者:
Metcalf,D
DOI:
10.1073/pnas.79.17.5374
发表时间:
1982
影响因子:
11.1
作者:
Ball,ED;Graziano,RF;Shen,L;Fanger,MW
通讯作者:
Fanger,MW