Lipopolysaccharide-induced expression of surfactant proteins A1 and A2 in human renal tubular epithelial cells.
Lipopolysaccharide-induced expression of surfactant proteins A1 and A2 in human renal tubular epithelial cells.
复制标题
脂多糖诱导人肾小管上皮细胞中表面活性蛋白 A1 和 A2 的表达。
DOI：
10.1186/1476-9255-10-2
复制
发表时间：
2013-01-12
期刊：
影响因子：
--
通讯作者：
Ding G
中科院分区：
文献类型：
--
作者：
Liu J;Hu F;Wang G;Zhou Q;Ding G
表面活性蛋白A（SP-A）由两个功能基因SP-A1和SP-A2编码，对于肺部的炎症过程和宿主防御是必需的。最近的研究表明SP-A在肺外表达。与肺相似，肾脏是暴露于外部病原体的器官。本研究评价了SP-A在肾脏中的表达和定位。在肾小管上皮细胞（HK-2）中观察脂多糖（LPS）对SP-A亚型表达的影响。用SP-A多克隆抗体进行免疫组织化学染色。RT-PCR也使用mRNA从正常人肾组织和HK-2细胞进行。SP-A1和SP-A2基因的表达通过基于PCR的RFLP分析、基因特异性扩增和RT-PCR产物的直接测序来确定。Western blot分析SP-A蛋白。HK-2细胞用不同浓度（0、0.1、1、2、5和10 μg/mL）的LPS处理8 h，并在不同时间点（0、2、4、8、16和24 h）用5 μg/mL的LPS处理。RT-PCR和Western blot检测LPS诱导的SP-A1和SP-A2 mRNA和蛋白表达。SP-A定位于近曲小管和远曲小管上皮细胞。HK-2细胞和人肾组织中SP-A1和SP-A2 mRNA和蛋白的表达在LPS处理后呈时间和剂量依赖性明显增加（P < 0.05）。人肾小管上皮细胞同时表达SP-A1和SP-A2基因，可能在肾脏的炎症调节中起重要作用。
Surfactant protein A (SP-A), encoded by two functional genes, SP-A1 and SP-A2, is essential for the inflammatory process and host defence in the lungs. Recent studies have demonstrated the extrapulmonary expression of SP-A. Similar to the lungs, the kidneys are organs exposed to external pathogens. The present study evaluated the expression and location of SP-A in the kidneys. The effect of lipopolysaccharide (LPS) on the expression of SP-A subtypes was also studied in renal tubular epithelial (HK-2) cells. Immunohistochemical staining was performed using polyclonal antibody against SP-A. RT-PCR was also performed using mRNA from normal human renal tissues and HK-2 cells. The expressions of the SP-A1 and SP-A2 genes were determined by PCR-based RFLP analysis, gene-specific amplification, and direct sequencing of RT-PCR products. Western blot was conducted to analyse the SP-A protein. HK-2 cells were treated with LPS at various concentrations (0, 0.1, 1, 2, 5, and 10 μg/mL) for 8 h and at 5 μg/mL at various time points (0, 2, 4, 8, 16, and 24 h). The LPS-induced expressions of SP-A1 and SP-A2 mRNA and protein were analysed by RT-PCR and Western blot. SP-A was localised in the renal tubular epithelial cells in the proximal and distal convoluted tubules. SP-A1 and SP-A2 mRNA and protein were expressed in HK-2 cells and human renal tissues, which were significantly increased in time- and dose-dependent manners after LPS treatment (P < 0.05). Human renal tubular epithelial cells can express both SP-A1 and SP-A2 genes, which may play important roles in the inflammatory modulation of the kidney.
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DOI：
10.1165/ajrcmb/6.4.446
发表时间：
1992-04-01
影响因子：
6.4
作者：
KATYAL, SL;SINGH, G;LOCKER, J
通讯作者：
LOCKER, J
DOI：
10.1165/rcmb.2002-0274oc
发表时间：
2003-11-01
影响因子：
6.4
作者：
Madsen, J;Tornoe, I;Holmskov, U
通讯作者：
Holmskov, U
DOI：
10.1152/ajplung.2001.280.2.l214
发表时间：
2001-02-01
影响因子：
4.9
作者：
Paananen, R;Glumoff, V;Hallman, M
通讯作者：
Hallman, M
影响因子：
3.5
作者：
Chuang, Chi-Yuan;Chen, Ta-Liang;Chen, Ruei-Ming
通讯作者：
Chen, Ruei-Ming
影响因子：
--
作者：
MCCORMICK, SM;BOGGARAM, V;MENDELSON, CR
通讯作者：
MENDELSON, CR