Role of β-catenin in post-meiotic male germ cell differentiation.
Role of β-catenin in post-meiotic male germ cell differentiation.
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β-catenin在脂肪生殖后生殖细胞分化中的作用。
DOI：
10.1371/journal.pone.0028039
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Rao MK
中科院分区：
文献类型：
--
作者：
Chang YF;Lee-Chang JS;Harris KY;Sinha-Hikim AP;Rao MK
虽然β-catenin信号转导在睾丸发育中的作用已经得到很好的证实，但对其在出生后睾丸生理学中的作用知之甚少。更少的是知道它在减数分裂后的生殖细胞发育和分化的作用。在此，我们报道β-catenin在小鼠减数分裂后的生殖细胞中高度表达，并在精子发生过程中发挥重要作用。精细胞特异性β-连环蛋白缺失导致精子数量显著减少，生殖细胞凋亡增加和生育力受损。此外，超微结构研究表明，减数分裂后生殖细胞中β-连环蛋白的缺失导致顶体缺陷、未成熟精子细胞的异常释放以及支持细胞与伸长精子细胞之间的粘附连接的破坏（顶端外质特化; ES）。这些缺陷可能是由于改变了表达的几个基因，据报道参与支持细胞-生殖细胞粘附和生殖细胞分化，如基因表达分析所揭示的。综上所述，我们的研究结果表明，β-连环蛋白是一个重要的分子连接，整合支持细胞-生殖细胞粘附与减数分裂后生殖细胞发育和成熟所必需的信号事件。由于β-catenin在Sertoli细胞中也高度表达，我们认为生殖细胞β-catenin复合物与Sertoli细胞上的β-catenin复合物在顶端ES表面的结合触发了调节减数分裂后生殖细胞分化的信号级联。
Though roles of β-catenin signaling during testis development have been well established, relatively little is known about its role in postnatal testicular physiology. Even less is known about its role in post-meiotic germ cell development and differentiation. Here, we report that β-catenin is highly expressed in post-meiotic germ cells and plays an important role during spermiogenesis in mice. Spermatid-specific deletion of β-catenin resulted in significantly reduced sperm count, increased germ cell apoptosis and impaired fertility. In addition, ultrastructural studies show that the loss of β-catenin in post-meiotic germ cells led to acrosomal defects, anomalous release of immature spermatids and disruption of adherens junctions between Sertoli cells and elongating spermatids (apical ectoplasmic specialization; ES). These defects are likely due to altered expression of several genes reportedly involved in Sertoli cell-germ cell adhesion and germ cell differentiation, as revealed by gene expression analysis. Taken together, our results suggest that β-catenin is an important molecular link that integrates Sertoli cell-germ cell adhesion with the signaling events essential for post-meiotic germ cell development and maturation. Since β-catenin is also highly expressed in the Sertoli cells, we propose that binding of germ cell β-catenin complex to β-catenin complex on Sertoli cell at the apical ES surface triggers a signaling cascade that regulates post-meiotic germ cell differentiation.
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基于 F-肌动蛋白的头部囊细胞延伸物粘附在成熟的精子细胞上，使它们保持紧密的束状，并防止它们在果蝇睾丸中过早释放。
影响因子：
5.4
作者：
Desai BS;Shirolikar S;Ray K
通讯作者：
Ray K
影响因子：
64.5
作者：
FRESHNEY, NW;RAWLINSON, L;SAKLATVALA, J
通讯作者：
SAKLATVALA, J
DOI：
10.1111/j.1600-0854.2009.00950.x
发表时间：
2009-09
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Guo X;Tu L;Gumper I;Plesken H;Novak EK;Chintala S;Swank RT;Pastores G;Torres P;Izumi T;Sun TT;Sabatini DD;Kreibich G
通讯作者：
Kreibich G
影响因子：
16
作者：
Centore RC;Havens CG;Manning AL;Li JM;Flynn RL;Tse A;Jin J;Dyson NJ;Walter JC;Zou L
通讯作者：
Zou L
影响因子：
2.7
作者：
Chang YF;Lee-Chang JS;Panneerdoss S;MacLean JA 2nd;Rao MK
通讯作者：
Rao MK