Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/FNIP2 Axis in Chicken Adipogenesis.
Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/FNIP2 Axis in Chicken Adipogenesis.
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DOI：
10.3389/fcell.2021.653798
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发表时间：
2021
影响因子：
5.5
通讯作者：
Luo Q
中科院分区：
文献类型：
--
作者：
Guo L;Chao X;Huang W;Li Z;Luan K;Ye M;Zhang S;Liu M;Li H;Luo W;Nie Q;Zhang X;Luo Q
脂质的生物合成是一个复杂的过程，受包括lncRNA在内的多种因素的调控。然而，lncRNA在鸡腹脂积累中的作用尚不清楚。本研究选取6只高腹脂率三黄鸡和6只低腹脂率三黄鸡的肝脏组织进行lncRNA和small RNA测序。总共有2，265个lncRNA，245个miRNAs和5，315个mRNAs被不同地表达。其中，1,136个差异表达基因在代谢过程中富集。共36个差异表达基因在代谢过程中富集，这些基因被认为是差异表达lncRNA的靶基因。此外，我们还发现8种不同表达的miRNAs可以靶向19种不同表达的基因。FNIP 2和PEX 5L在一个顺式调控网络和一个不同表达的miRNA靶点关系网络中共享。LncRNA-FNIP 2/miR-24- 3 p/FNIP 2轴被认为是可能参与脂质合成的潜在候选者。实验上，阐明了lncRNA-FNIP 2/miR-24- 3 p/FNIP 2轴的客观存在，验证了lncRNA-FNIP 2/miR-24- 3 p/FNIP 2轴对合成的调控作用。总之，我们的研究揭示了一个潜在的新的调控机制，lncRNA-FNIP 2/miR-24- 3 p/FNIP 2轴被认为参与了鸡肝脏脂肪形成过程中的脂质合成。
Lipid biosynthesis is a complex process, which is regulated by multiple factors including lncRNA. However, the role of lncRNA in chicken abdominal fat accumulation is still unclear. In this research, we collected liver tissues from six high abdominal fat rate Sanhuang broilers and six low abdominal fat rate Sanhuang broilers to perform lncRNA sequencing and small RNA sequencing. A total of 2,265 lncRNAs, 245 miRNAs, and 5,315 mRNAs were differently expressed. Among of them, 1,136 differently expressed genes were enriched in the metabolic process. A total of 36 differently expressed genes, which were considered as differently expressed lncRNAs’ targets, were enriched in the metabolic process. In addition, we also found out that eight differently expressed miRNAs could target 19 differently expressed genes. FNIP2 and PEX5L were shared in a cis-regulatory network and a differently expressed miRNA target relationship network. LncRNA-FNIP2/miR-24-3p/FNIP2 axis was considered as a potential candidate that may participate in lipid synthesis. Experimentally, the objective reality of lncRNA-FNIP2/miR-24-3p/FNIP2 axis was clarified and the regulation effect of lncRNA-FNIP2/miR-24-3p/FNIP2 axis on synthesis was validated. In brief, our study reveals a potential novel regulatory mechanism that lncRNA-FNIP2/miR-24-3p/FNIP2 axis was considered as being involved in lipid synthesis during chicken adipogenesis in liver.
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影响因子：
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影响因子：
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DOI：
10.1016/j.bbalip.2011.09.017
发表时间：
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期刊：
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影响因子：
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作者：
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