Increased susceptibility to oral Trichuris muris infection in the specific absence of CXCR5(+) CD11c(+) cells.
Increased susceptibility to oral Trichuris muris infection in the specific absence of CXCR5(+) CD11c(+) cells.
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DOI：
10.1111/pim.12566
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发表时间：
2018-08
影响因子：
2.2
通讯作者：
Mabbott NA
中科院分区：
文献类型：
--
作者：
Bradford BM;Donaldson DS;Forman R;Else KJ;Mabbott NA
鼠鞭虫是一种天然的小鼠蠕虫病原体，其特异性地在盲肠和近端结肠中建立感染。耐药小鼠品系中小鼠毛滴虫的快速排出与诱导保护性辅助性T细胞2型（Th 2）极化免疫应答相关。相反，易感小鼠品系对鼠弓形虫感染产生不适当的Th 1应答。间质滤泡树突状细胞表达趋化因子CXCL 13吸引表达CXCR 5的细胞朝向B细胞滤泡。先前使用复杂体内耗竭模型的研究表明，表达CXCR 5的常规树突状细胞（cDC）有助于调节Th 2极化反应的诱导。在此，使用具有特异性限制于CD 11 c+细胞的CXCR 5缺陷的转基因小鼠来确定CD 11 c+细胞（例如cDC）上CXCR 5的特异性缺失是否会通过影响Th 1/Th 2平衡来影响对口服鼠弓形虫感染的易感性。我们发现，与对照小鼠相比，那些在CD 11 c+细胞上缺乏CXCR 5表达的小鼠未能清除鼠弓形虫感染，并产生了细胞因子和抗体应答，这表明Th 1/Th 2平衡受到干扰，IFN-γ表达增强。这些数据表明表达CXCR 5的CD 11 c+细胞（如cDC）在对口服鼠弓形虫感染的免疫中发挥重要作用。
Trichuris muris is a natural mouse helminth pathogen which establishes infection specifically in the caecum and proximal colon. The rapid expulsion of T. muris in resistant mouse strains is associated with the induction of a protective T helper cell type 2 (Th2)‐polarized immune response. Susceptible mouse strains, in contrast, mount an inappropriate Th1 response to T. muris infection. Expression of the chemokine CXCL13 by stromal follicular dendritic cells attracts CXCR5‐expressing cells towards the B‐cell follicles. Previous studies using a complex in vivo depletion model have suggested that CXCR5‐expressing conventional dendritic cells (cDC) help regulate the induction of Th2‐polarized responses. Here, transgenic mice with CXCR5 deficiency specifically restricted to CD11c+ cells were used to determine whether the specific absence CXCR5 on CD11c+ cells such as cDC would influence susceptibility to oral T. muris infection by affecting the Th1/Th2 balance. We show that in contrast to control mice, those which lacked CXCR5 expression on CD11c+ cells failed to clear T. muris infection and developed cytokine and antibody responses that suggested a disturbed Th1/Th2 balance with enhanced IFN‐γ expression. These data suggest an important role of CXCR5‐expressing CD11c+ cells such as cDC in immunity to oral T. muris infection.
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影响因子：
5.4
作者：
Bradford BM;Reizis B;Mabbott NA
通讯作者：
Mabbott NA
影响因子：
2.2
作者：
Inman, CF;Rees, LEN;Bailey, M
通讯作者：
Bailey, M
影响因子：
32.4
作者：
Jung, S;Unutmaz, D;Lang, RA
通讯作者：
Lang, RA
影响因子：
2.8
作者：
Bradford, Barry M.;Sester, David P.;Mabbott, Neil A.
通讯作者：
Mabbott, Neil A.
DOI：
10.1084/jem.20062648
发表时间：
2007-07-09
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Caton ML;Smith-Raska MR;Reizis B
通讯作者：
Reizis B