Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation.

Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation.
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DOI:
10.1007/s13361-015-1088-z
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发表时间:
2015-07
影响因子:
3.2
通讯作者:
Breuker, Kathrin
Breuker, Kathrin
中科院分区:
化学3区
文献类型:
--
作者:
Schennach, Moritz;Breuker, Kathrin

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在凝聚相,X射线晶体学和核磁共振光谱学,原子详细的蛋白质结构的研究方法,最近已被补充的新技术,几乎或完全去溶剂化的蛋白质结构在气相实验中探测。电子捕获解离(ECD)是其中唯一的,因为它提供了残基特异性,虽然间接,结构信息。在这篇批判性洞察文章中,我们讨论了ECD用于气态蛋白质离子结构探测的发展,其潜力和局限性。ᅟ
The established methods for the study of atom-detailed protein structure in the condensed phases, X-ray crystallography and nuclear magnetic resonance spectroscopy, have recently been complemented by new techniques by which nearly or fully desolvated protein structures are probed in gas-phase experiments. Electron capture dissociation (ECD) is unique among these as it provides residue-specific, although indirect, structural information. In this Critical Insight article, we discuss the development of ECD for the structural probing of gaseous protein ions, its potential, and limitations. ᅟ
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