Sequence analysis of Arthrospira maxima based on fosmid library
Sequence analysis of Arthrospira maxima based on fosmid library
复制标题
基于fosmid文库的极大节旋藻序列分析
DOI：
10.1007/s10811-006-9140-9
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发表时间：
2007-02
影响因子：
3.3
通讯作者：
Ling, Na
中科院分区：
文献类型：
--
作者：
Zhang, Xuecheng;Wang, Guangce;Mo, Zhaolan;Liu, Wei;Mao, Yunxiang;Ling, Na
节旋藻（Arthrospira，Spirulina）是一种重要的蓝藻，不仅具有营养价值，而且具有独特的生物学特性。构建了包含4300个克隆的极大节旋藻FACHB 438无偏fosmid文库。插入片段大小分布在15.5 ~ 48.9kb之间，平均大小为37.6kb。重组率为100%。因此，该文库相当于节旋藻基因组大小5.4 Mb的29.9当量。从文库中随机抽取719个样本克隆，获得602个序列，包含307,547个碱基，占全基因组的5.70%。A.最大值没有强烈的偏差。密码子第一位的GC含量（46.9%）高于第二位（39.8%）和第三位（45.5%）。基因组GC含量为43.6%。其中287个（47.7%）与已知基因高度相似，63个（10.5%）与假设基因高度相似，252个（41.8%）与已知基因高度相似。根据不同的生物学功能，将指定的基因分为22类。值得注意的是，25个编码逆转录酶的序列（4.2%）的高存在表明RT基因在A.可能在进化史和代谢调控中起重要作用。此外，编码ATP结合盒转运系统和双组分信号转导系统的序列分别是第二和第三最常见的基因。这些基因组特征为该生物体适应高浓度碳酸氢盐和高pH环境的机制提供了一些线索。
Arthrospira (Spirulina) (Setchell & Gardner) is an important cyanobacterium not only in its nutritional potential but in its special biological characteristics. An unbiased fosmid library of Arthrospira maxima FACHB438 that contains 4300 clones was constructed. The size distribution of insert fragments is from 15.5 to 48.9 kb and the average size is 37.6 kb. The recombination frequency is 100%. Therefore the library is 29.9 equivalents to the Arthrospira genome size of 5.4 Mb. A total of 719 sample clones were randomly chosen from the library and 602 available sequences, which consisted of 307,547 bases, covering 5.70% of the whole genome. The codon usage of A. maxima was not strongly biased. GC content at the first position of codons (46.9%) was higher than the second (39.8%) and the third (45.5%) positions. GC content of the genome was 43.6%. Of these sequences, 287 (47.7%) showed high similarities to known genes, 63 (10.5%) to hypothetical genes and the remaining 252 (41.8%) had no significant similarities. The assigned genes were classified into 22 categories with respect to different biological roles. Remarkably, the high presence of 25 sequences (4.2%) encoding reverse transcriptase indicates the RT gene may have multiple copies in the A. maxima genome and might play an important role in the evolutionary history and metabolic regulation. In addition, the sequences encoding the ATP-binding cassette transport system and the two-component signal transduction system were the second and third most frequent genes, respectively. These genomic features provide some clues as to the mechanisms by which this organism adapts to the high concentration of bicarbonate and to the high pH environment.
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影响因子：
--
作者：
Kojima, Hiroyuki;Qin Song;Yano, Shin-ichi
通讯作者：
Yano, Shin-ichi
影响因子：
2.1
作者：
L. Lanfaloni;M. Trinei;M. Russo;C. Gualerzi
通讯作者：
L. Lanfaloni;M. Trinei;M. Russo;C. Gualerzi
DOI：
10.1201/9781482272970
发表时间：
1997-05
期刊：
--
影响因子：
--
作者：
A. Vonshak
通讯作者：
A. Vonshak
DOI：
10.1073/pnas.96.2.784
发表时间：
1999-01-19
影响因子：
11.1
作者：
Reumann, S;Davila-Aponte, J;Keegstra, K
通讯作者：
Keegstra, K
影响因子：
3.2
作者：
García-Domínguez, M;Muro-Pastor, MI;Florencio, FJ
通讯作者：
Florencio, FJ