Lack of evidence for xenotropic murine leukemia virus-related virus(XMRV) in German prostate cancer patients.
Lack of evidence for xenotropic murine leukemia virus-related virus(XMRV) in German prostate cancer patients.
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DOI：
10.1186/1742-4690-6-92
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发表时间：
2009-10-16
期刊：
影响因子：
3.3
通讯作者：
Bannert N
中科院分区：
文献类型：
--
作者：
Hohn O;Krause H;Barbarotto P;Niederstadt L;Beimforde N;Denner J;Miller K;Kurth R;Bannert N
最近发现一种名为异嗜性小鼠白血病病毒相关病毒（XMRV）的新型γ逆转录病毒，并发现其在RNaseL基因中携带纯合R462 Q突变的美国患者的前列腺肿瘤样本中的患病率为40%。这种突变损害了先天抗病毒I型干扰素途径的功能，并且是前列腺癌的已知易感因素。在这里，我们试图测量XMRV在德国前列腺癌病例中的患病率，并确定是否存在与R462 Q多态性的类似关联。通过实时PCR对589例前列腺肿瘤样本的RNaseL突变进行基因分型。使用高度灵敏的巢式PCR和RT-PCR方法筛选来自这些患者的DNA和RNA样品中是否存在XMRV特异性gag序列。此外，使用新开发的ELISA测定法对来自前列腺肿瘤患者的146份血清样品进行XMRV Gag和Env抗体测试。与早期数据一致，12.9%（76个样本）显示为QQ基因型。然而，XMRV特异性序列在DNA和RNA水平均未检测到。与该结果一致，从前列腺癌患者分析的血清均不含XMRV特异性抗体。我们的研究结果表明，在德国的前列腺肿瘤患者中，XMRV的患病率要低得多（甚至完全没有）。其中一个可能的原因可能是XMRV感染的地理限制。
A novel gammaretrovirus named xenotropic murine leukemia virus-related virus (XMRV) has been recently identified and found to have a prevalence of 40% in prostate tumor samples from American patients carrying a homozygous R462Q mutation in the RNaseL gene. This mutation impairs the function of the innate antiviral type I interferon pathway and is a known susceptibility factor for prostate cancer. Here, we attempt to measure the prevalence of XMRV in prostate cancer cases in Germany and determine whether an analogous association with the R462Q polymorphism exists. 589 prostate tumor samples were genotyped by real-time PCR with regard to the RNaseL mutation. DNA and RNA samples from these patients were screened for the presence of XMRV-specific gag sequences using a highly sensitive nested PCR and RT-PCR approach. Furthermore, 146 sera samples from prostate tumor patients were tested for XMRV Gag and Env antibodies using a newly developed ELISA assay. In agreement with earlier data, 12.9% (76 samples) were shown to be of the QQ genotype. However, XMRV specific sequences were detected at neither the DNA nor the RNA level. Consistent with this result, none of the sera analyzed from prostate cancer patients contained XMRV-specific antibodies. Our results indicate a much lower prevalence (or even complete absence) of XMRV in prostate tumor patients in Germany. One possible reason for this could be a geographically restricted incidence of XMRV infections.
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