Divergent Antiviral Mechanisms of Two Viperin Homeologs in a Recurrent Polyploid Fish.
Divergent Antiviral Mechanisms of Two Viperin Homeologs in a Recurrent Polyploid Fish.
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轮回多倍体鱼中两种蝰蛇蛋白同源物的不同抗病毒机制
DOI：
10.3389/fimmu.2021.702971
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发表时间：
2021
影响因子：
7.3
通讯作者：
Gui JF
中科院分区：
文献类型：
--
作者：
Mou CY;Li S;Lu LF;Wang Y;Yu P;Li Z;Tong JF;Zhang QY;Wang ZW;Zhang XJ;Wang GX;Zhou L;Gui JF
多倍体和随后的二倍体化为进化创新和适应提供了基因组机会。轮回多倍体重复基因进化命运的研究严重滞后于二倍体古多倍体。此外，蝰蛇蛋白在鱼类中的抗病毒机制在很大程度上仍不清楚。在这里，我们阐述了不同的抗病毒机制的两个毒蛇同源（Cgviperin-A和Cgviperin-B）在同源异源六倍体异育银鲫（Carassius gibelio）。首先，Cgviperin-A和Cgviperin-B在异育银鲫成体组织中表现出差异性和偏向性表达模式。随后，通过免疫共沉淀（Co-IP）筛选分析，发现CgViperin-A和CgViperin-B都与鲫鱼（C. auratus）疱疹病毒（CaHV）开放阅读框46右（ORF 46 R）蛋白，其是宿主干扰素（IFN）产生的负性疱疹病毒调节剂，并通过减少K63连接的泛素化来促进ORF 46 R的蛋白酶体降解。此外，CgViperin-B还通过自噬体途径介导ORF 46 R降解，这在CgViperin-A中不存在。此外，我们还发现CgViperin-A和CgViperin-B的N端α螺旋结构域是其在内质网定位所必需的，而CgViperin-A和CgViperin-B的C端结构域是其与ORF 46 R降解相互作用所必需的。因此，目前的研究结果澄清了重复的蝰蛇蛋白同源物在一个经常性的多倍体鱼不同的抗病毒机制，这将揭示硬骨鱼重复基因的进化。
Polyploidy and subsequent diploidization provide genomic opportunities for evolutionary innovations and adaptation. The researches on duplicated gene evolutionary fates in recurrent polyploids have seriously lagged behind that in paleopolyploids with diploidized genomes. Moreover, the antiviral mechanisms of Viperin remain largely unclear in fish. Here, we elaborate the distinct antiviral mechanisms of two viperin homeologs (Cgviperin-A and Cgviperin-B) in auto-allo-hexaploid gibel carp (Carassius gibelio). First, Cgviperin-A and Cgviperin-B showed differential and biased expression patterns in gibel carp adult tissues. Subsequently, using co-immunoprecipitation (Co-IP) screening analysis, both CgViperin-A and CgViperin-B were found to interact with crucian carp (C. auratus) herpesvirus (CaHV) open reading frame 46 right (ORF46R) protein, a negative herpesvirus regulator of host interferon (IFN) production, and to promote the proteasomal degradation of ORF46R via decreasing K63-linked ubiquitination. Additionally, CgViperin-B also mediated ORF46R degradation through autophagosome pathway, which was absent in CgViperin-A. Moreover, we found that the N-terminal α-helix domain was necessary for the localization of CgViperin-A and CgViperin-B at the endoplasmic reticulum (ER), and the C-terminal domain of CgViperin-A and CgViperin-B was indispensable for the interaction with degradation of ORF46R. Therefore, the current findings clarify the divergent antiviral mechanisms of the duplicated viperin homeologs in a recurrent polyploid fish, which will shed light on the evolution of teleost duplicated genes.
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影响因子：
7.3
作者：
Eslamloo, Khalil;Ghorbani, Atefeh;Rise, Matthew L.
通讯作者：
Rise, Matthew L.
DOI：
10.1073/pnas.0911679106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Hinson, Ella R.;Cresswell, Peter
通讯作者：
Cresswell, Peter
多倍体银鲫克隆多样性和双重繁殖模式的遗传基础及育种应用
影响因子：
9.1
作者：
Gui JianFang;Zhou Li
通讯作者：
Zhou Li
影响因子：
4.4
作者：
Gao FX;Wang Y;Zhang QY;Mou CY;Li Z;Deng YS;Zhou L;Gui JF
通讯作者：
Gui JF
DOI：
10.1074/jbc.m807261200
发表时间：
2009-02-13
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hinson ER;Cresswell P
通讯作者：
Cresswell P