Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes.
Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes.
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DOI：
10.1186/s12864-017-3945-6
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发表时间：
2017-07-24
期刊：
影响因子：
4.4
通讯作者：
Gui JF
中科院分区：
文献类型：
--
作者：
Gao FX;Wang Y;Zhang QY;Mou CY;Li Z;Deng YS;Zhou L;Gui JF
鲫鱼是中国重要的水产养殖品种，一种名为鲫鱼疱疹病毒（Carassius auratus herpesvirus, CaHV）的疱疹病毒阻碍了其养殖发展。各种异育银鲫的雌性生殖克隆已被鉴定或培育出来，其中一些已被用作水产养殖品种，但它们对疱疹病毒的抗性及其潜在机制尚不清楚。为了揭示它们的易感性差异，我们首先对3个异育银鲫雌化无性系（主要品种A+、候选品种F和野生无性系h）进行了疱疹病毒攻毒实验，3个无性系对CaHV具有明显的抗性。从A+、F和H克隆患病个体与对照个体的比较转录组中分别鉴定出8772、8679和10982个差异表达单基因（deu）。综合分析所有三个克隆的共享deu显示了对疱疹病毒感染的共同防御途径，激活IFN系统并抑制补体。KEGG途径分析显示，不同克隆特异性改变的deu对疱疹病毒感染有不同的免疫反应。从克隆H中鉴定出的KEGG免疫相关通路（如“趋化因子信号通路”、“toll样受体信号通路”等）中的DEU数显著高于克隆A+和克隆f。抗性克隆H中Mx1、viperin、PKR等ifn相关基因的增加量高于其他基因。IFNphi3、IFI44-like和Gig2在F克隆中表达量最高，IRF1仅在A+易感克隆中表达量增加。与抗性克隆H的强免疫防御相比，易感克隆A+的凋亡或死亡相关基因显著上调，说明A+克隆未能抵抗病毒的攻击，明显诱导了细胞凋亡或死亡。本研究首次尝试利用综合转录组技术筛选三种雌化异育银鲫对疱疹病毒感染的不同抗性和免疫反应。这些从易感和抗性克隆中鉴定出的差异deu、免疫相关途径和IFN系统基因，将有利于异育银鲫的标记辅助选择育种或基于分子模块的抗性育种。本文的在线版本（doi:10.1186/s12864-017-3945-6）包含补充材料，可供授权用户使用。
Gibel carp is an important aquaculture species in China, and a herpesvirus, called as Carassius auratus herpesvirus (CaHV), has hampered the aquaculture development. Diverse gynogenetic clones of gibel carp have been identified or created, and some of them have been used as aquaculture varieties, but their resistances to herpesvirus and the underlying mechanism remain unknown. To reveal their susceptibility differences, we firstly performed herpesvirus challenge experiments in three gynogenetic clones of gibel carp, including the leading variety clone A+, candidate variety clone F and wild clone H. Three clones showed distinct resistances to CaHV. Moreover, 8772, 8679 and 10,982 differentially expressed unigenes (DEUs) were identified from comparative transcriptomes between diseased individuals and control individuals of clone A+, F and H, respectively. Comprehensive analysis of the shared DEUs in all three clones displayed common defense pathways to the herpesvirus infection, activating IFN system and suppressing complements. KEGG pathway analysis of specifically changed DEUs in respective clones revealed distinct immune responses to the herpesvirus infection. The DEU numbers identified from clone H in KEGG immune-related pathways, such as “chemokine signaling pathway”, “Toll-like receptor signaling pathway” and others, were remarkably much more than those from clone A+ and F. Several IFN-related genes, including Mx1, viperin, PKR and others, showed higher increases in the resistant clone H than that in the others. IFNphi3, IFI44-like and Gig2 displayed the highest expression in clone F and IRF1 uniquely increased in susceptible clone A+. In contrast to strong immune defense in resistant clone H, susceptible clone A+ showed remarkable up-regulation of genes related to apoptosis or death, indicating that clone A+ failed to resist virus offensive and evidently induced apoptosis or death. Our study is the first attempt to screen distinct resistances and immune responses of three gynogenetic gibel carp clones to herpesvirus infection by comprehensive transcriptomes. These differential DEUs, immune-related pathways and IFN system genes identified from susceptible and resistant clones will be beneficial to marker-assisted selection (MAS) breeding or molecular module-based resistance breeding in gibel carp. The online version of this article (doi:10.1186/s12864-017-3945-6) contains supplementary material, which is available to authorized users.
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