Improving axial resolution in Structured Illumination Microscopy using deep learning.
Improving axial resolution in Structured Illumination Microscopy using deep learning.
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DOI:
10.1098/rsta.2020.0298
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发表时间:
2021-06-14
期刊:
影响因子:
--
通讯作者:
Neil MAA
中科院分区:
文献类型:
--
作者:
Boland MA;Cohen EAK;Flaxman SR;Neil MAA
Structured Illumination Microscopy (SIM) is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep learning models, we demonstrate a method to reconstruct 3D SIM image stacks with twice the axial resolution attainable through conventional SIM reconstructions. We further demonstrate our method is robust to noise and evaluate it against two-point cases and axial gratings. Finally, we discuss potential adaptions of the method to further improve resolution. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.
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