Small molecules released from islets of Langerhans determined by liquid chromatography - mass spectrometry.
Small molecules released from islets of Langerhans determined by liquid chromatography - mass spectrometry.
复制标题
DOI：
10.1039/d2ay00402j
复制
发表时间：
2022-06-01
影响因子：
3.1
通讯作者：
Roper, Michael G.
中科院分区：
文献类型：
--
作者：
Ogunkunle, Emmanuel O.;Donohue, Matthew J.;Steyer, Daniel J.;Adeoye, Damilola, I;Eaton, Wesley J.;Roper, Michael G.
胰岛是胰腺内分泌组织，分泌激素维持血糖稳态。各种小分子，包括经典的神经递质也从胰岛中释放出来。虽然大多数这些小分子的作用尚不清楚，但一些小分子被假设在胰岛生理学中发挥关键作用。为了更好地了解它们对胰岛功能的作用，开发了液相色谱-串联质谱（LC-MS/MS）方法来分离和定量从胰岛释放的39种小分子。分析物分子的苯甲酰氯衍生化用于赋予保留和促进电喷雾电离效率。在2.1 × 150 mm的色谱柱上，以2.7 μm核壳结构的C18颗粒为填充剂进行分离。校准曲线显示浓度和分析物响应之间具有良好的线性，分析物响应的相对标准偏差低于15%，检测限为0.01-40 nM。该方法被应用于检查小鼠和人类胰岛静态孵育和葡萄糖灌注后释放的小分子。结果显示，随着葡萄糖浓度的增加，大多数分析物的分泌速率降低。发现人类胰岛的分泌率高于鼠胰岛。这种方法将有助于理解小分子在生物系统中的作用。胰岛是胰腺内分泌组织，分泌激素维持血糖稳态。
Islets of Langerhans are the endocrine tissue within the pancreas that secrete hormones for maintenance of blood glucose homeostasis. A variety of small molecules including classical neurotransmitters are also released from islets. While the roles of most of these small molecules are unknown, some have been hypothesized to play a critical role in islet physiology. To better understand their role on islet function, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to separate and quantify 39 small molecules released from islets. Benzoyl chloride derivatization of analyte molecules was used to impart retention and facilitate electrospray ionization efficiency. Separation was achieved on a 2.1 × 150 mm column packed with 2.7 μm core–shell C18 particles. Calibration curves showed excellent linearity between the concentration and analyte response, with relative standard deviations of the analyte responses below 15% and limits of detection from 0.01–40 nM. The method was applied to examine small molecules released from murine and human islets of Langerhans after static incubation and perfusion with glucose. Results showed a decrease in secretion rates with increasing glucose concentration for most of the analytes. Secretion rates were found to be higher in human islets compared to their murine counterpart. This method will be useful in understanding the roles of small molecules in biological systems. Islets of Langerhans are the endocrine tissue within the pancreas that secrete hormones for maintenance of blood glucose homeostasis.
登录
查看更多内容
影响因子：
16.2
作者：
Bruno, G;Runzo, C;Pagano, G
通讯作者：
Pagano, G
DOI：
10.1039/d1ay00703c
发表时间：
2021-08-28
期刊：
Analytical methods : advancing methods and applications
影响因子：
--
作者：
Eaton WJ;Roper MG
通讯作者：
Roper MG
影响因子：
7.4
作者：
Lomasney, Anna R.;Yi, Lian;Roper, Michael G.
通讯作者：
Roper, Michael G.
影响因子：
20.8
作者：
Menegaz, Danusa;Hagan, D. Walker;Phelps, Edward A.
通讯作者：
Phelps, Edward A.
影响因子：
7.4
作者：
Lee, Woong Hee;Ngernsutivorakul, Thitaphat;Mabrouk, Omar S.;Wong, Jenny-Marie T.;Dugan, Colleen E.;Pappas, Samuel S.;Yoon, Hyeun Joong;Kennedy, Robert T.
通讯作者：
Kennedy, Robert T.