Microfabrication and in Vivo Performance of a Microdialysis Probe with Embedded Membrane.
Microfabrication and in Vivo Performance of a Microdialysis Probe with Embedded Membrane.
复制标题
DOI：
10.1021/acs.analchem.5b03541
复制
发表时间：
2016-01-19
影响因子：
7.4
通讯作者：
Kennedy, Robert T.
中科院分区：
文献类型：
--
作者：
Lee, Woong Hee;Ngernsutivorakul, Thitaphat;Mabrouk, Omar S.;Wong, Jenny-Marie T.;Dugan, Colleen E.;Pappas, Samuel S.;Yoon, Hyeun Joong;Kennedy, Robert T.
微透析取样是体内神经化学监测的重要工具。传统的透析探头直径超过220 μm，设计灵活性有限，因为它们是通过使用预成型膜组装而成的。探头尺寸限制了空间分辨率，并决定了探头插入导致的组织损伤量。为了克服这些限制，我们已经开发出一种方法，在硅微制造探针是45 μm厚× 180 μm宽。探针包含一个深30 μm ×宽60 μm的埋入式U形通道，并终止于用于外部连接的端口。4 mm长的探针覆盖有5 μm厚的纳米多孔膜。通过多孔氧化铝层的深反应离子蚀刻的膜微制造。微加工探针的横截面积比最小的常规微透析探针的横截面积小79%。对于各种小分子，探针在100 nL/min灌注速率下产生2-7%的相对回收率。通过从活体大鼠纹状体取样，成功地在体内测试了探针。在腹膜内注射5 mg/ kg安非他明之前和之后，以20 min间隔（2 μL）收集组分。通过液相色谱-质谱法分析馏分显示可靠的检测13神经化学物质，包括多巴胺和乙酰胆碱，在基础条件。安非他明引起多巴胺增加43倍，这一结果与同一动物的传统透析探针几乎相同。微加工探针具有比传统探针更高的空间分辨率和更少的组织破坏的采样潜力。还可以通过集成其他组件（例如电极、光学器件和附加通道）来向探头添加功能。
Microdialysis sampling is an essential tool for in vivo neurochemical monitoring. Conventional dialysis probes are over 220 μm in diameter and have limited flexibility in design because they are made by assembly using preformed membranes. The probe size constrains spatial resolution and governs the amount of tissue damaged caused by probe insertion. To overcome these limitations, we have developed a method to microfabricate probes in Si that are 45 μm thick × 180 μm wide. The probes contain a buried, U-shaped channel that is 30 μm deep × 60 μm wide and terminates in ports for external connection. A 4 mm length of the probe is covered with a 5 μm thick nanoporous membrane. The membrane was microfabricated by deep reactive ion etching through a porous aluminum oxide layer. The microfabricated probe has cross-sectional area that is 79% less than that of the smallest conventional microdialysis probes. The probes yield 2–7% relative recovery at 100 nL/min perfusion rate for a variety of small molecules. The probe was successfully tested in vivo by sampling from the striatum of live rats. Fractions were collected at 20 min intervals (2 μL) before and after an intraperitoneal injection of 5 mg/ kg amphetamine. Analysis of fractions by liquid chromatography-mass spectrometry revealed reliable detection of 13 neurochemicals, including dopamine and acetylcholine, at basal conditions. Amphetamine evoked a 43-fold rise in dopamine, a result nearly identical to a conventional dialysis probe in the same animal. The microfabricated probes have potential for sampling with higher spatial resolution and less tissue disruption than conventional probes. It may also be possible to add functionality to the probes by integrating other components, such as electrodes, optics, and additional channels.
登录
查看更多内容
影响因子：
4.7
作者：
Kanamori, K;Ross, BD
通讯作者：
Ross, BD
影响因子：
5
作者：
Jina, Arvind;Tierney, Michael J;Christiansen, Mark
通讯作者：
Christiansen, Mark
影响因子：
2.7
作者：
Chandrasekaran, S;Brazzle, JD;Frazier, AB
通讯作者：
Frazier, AB
影响因子：
7.4
作者：
CHURCH, WH;JUSTICE, JB
通讯作者：
JUSTICE, JB
影响因子：
6.1
作者：
Leichle, Thierry;Bourrier, David
通讯作者：
Bourrier, David