Carbon Nanodots Inhibit Oxidized Low Density Lipoprotein-Induced Injury and Monocyte Adhesion to Endothelial Cells Through Scavenging Reactive Oxygen Species.
Carbon Nanodots Inhibit Oxidized Low Density Lipoprotein-Induced Injury and Monocyte Adhesion to Endothelial Cells Through Scavenging Reactive Oxygen Species.
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DOI：
10.1166/jbn.2021.3125
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发表时间：
2021-08-01
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
氧化低密度脂蛋白（Ox-LDL）是炎症和动脉粥样硬化的已知生物标志物，是全球死亡的主要原因。作为一种新型的纳米材料，碳纳米点（CND）在生物成像、诊断和药物输送等领域有着广泛的应用。然而，目前还没有关于这些CND如何影响心血管系统的报道，特别是它们在介导内皮炎症功能障碍中的潜力。本研究检测了CND对Ox-LDL介导的内皮功能障碍的影响。在人微血管内皮细胞（HMEC-1）中，CND显著抑制Ox-LDL介导的单核细胞与人微血管内皮细胞（HMEC-1）的粘附。CND显著抑制Ox-LDL介导的单核细胞与内皮细胞的粘附，这是动脉粥样硬化发展中的重要步骤。此外，CND显着抑制OxLDL诱导的白细胞介素-8（IL-8）的表达，这是一种对单核细胞粘附至内皮细胞至关重要的细胞因子。这些结果表明CND具有抗炎特性。CND还保护细胞免受Ox-LDL诱导的细胞毒性。电子顺磁共振（EPR）光谱研究表明，直接活性氧清除CNDs。这一结果表明，CND的抗炎特性最有可能是由于其直接清除活性氧。涉及小鼠的动物研究未显示CND组和对照组之间的任何形态学或物理变化。我们的研究提供了CND在减少HMEC-1中Ox-LDL介导的炎症和细胞毒性方面的潜力的证据。
Oxidized low density lipoprotein (Ox-LDL) is a known biomarker of inflammation and atherosclerosis, a leading cause of death worldwide. As a new class of nanomaterials, carbon nanodots (CNDs) are widely used in bioimaging, diagnostics, and drug delivery. However, there is no current report on how these CNDs affect the cardiovascular system, particularly their potential in mediating endothelial inflammatory dysfunction. This study examined effects of CNDs on Ox-LDL-mediated endothelial dysfunction. CNDs significantly inhibited Ox-LDL-mediated adhesion of monocytes to human microvascular endothelial cells (HMEC-1), in human microvascular endothelial cells (HMEC-1). CNDs significantly inhibited Ox-LDL-mediated adhesion of monocytes to endothelial cells, which is an essential step in the development of atherosclerosis. Further, CNDs significantly inhibited OxLDL-induced expression of interleukin-8 (IL-8), a vital cytokine on monocyte adhesion to the endothelial cells. These results demonstrate CNDs possess anti-inflammatory properties. CNDs also protect cells against Ox-LDL-induced cytotoxicity. Electron paramagnetic resonance (EPR) spectroscopy studies demonstrated direct reactive oxygen species-scavenging by CNDs. This result indicates that the anti-inflammatory properties of CNDs are most likely due to their direct scavenging of reactive oxygen species. Animal studies involving mice did not show any morphological or physical changes between the CNDs and control groups. Our study provides evidence of potential of CNDs in reducing Ox-LDL-mediated inflammation and cytotoxicity in HMEC-1.
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DOI：
10.3390/nano11051247
发表时间：
2021-05-10
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Belperain S;Kang ZY;Dunphy A;Priebe B;Chiu NHL;Jia Z
通讯作者：
Jia Z
基于碳纳米点和聚合物功能化有机-无机钙钛矿的高特异性光电化学平台，用于胆固醇传感
影响因子：
6.1
作者：
Chen, Lu;Song, Minxia;Hu, Xiao-Ya
通讯作者：
Hu, Xiao-Ya
影响因子：
4.3
作者：
Jia, Zhenquan;Zhu, Hong;Misra, Hara P.
通讯作者：
Misra, Hara P.
影响因子：
5
作者：
Bar-Eli, M
通讯作者：
Bar-Eli, M
影响因子：
6.5
作者：
ADES, EW;CANDAL, FJ;LAWLEY, TJ
通讯作者：
LAWLEY, TJ