Repairing Volumetric Muscle Loss in the Ovine Peroneus Tertius Following a 6-Month Recovery.
Repairing Volumetric Muscle Loss in the Ovine Peroneus Tertius Following a 6-Month Recovery.
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修复 6 个月恢复后绵羊腓骨第三肌的体积肌肉损失。
DOI：
10.1089/ten.tea.2021.0187
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发表时间：
2022
影响因子：
--
通讯作者：
Larkin,LisaM
中科院分区：
文献类型：
--
作者：
Rodriguez,BrittanyL;Novakova,StoynaS;Vega-Soto,EmmanuelE;Nutter,GenevieveP;Macpherson,PeterCD;Larkin,LisaM
组织工程化骨骼肌是治疗肌肉萎缩的一种新方法。我们的实验室已经开发了组织工程骨骼肌单位（SMU）和工程神经导管（ENC），并将其模块化扩展到临床相关的大小，用于在大型动物（绵羊）模型中治疗VML。在先前的研究中，我们评估了SMU和ENC在3个月恢复期后治疗30% VML损伤的绵羊腓骨第三肌的效果。本研究的目的是扩展我们3个月的研究，并评估SMU和ENC在6个月恢复期后恢复肌肉功能的作用。植入后6个月，我们发现SMU修复组（VML+SMU和VML+SMU+ENC）的肌肉质量恢复到与未受伤的对侧肌肉在统计学上无法区分的水平。相比之下，仅VML组的肌肉质量显著小于用SMU修复的组。6个月后恢复VML，最大强直力是显着低于所有VML损伤组相比，未受伤的对侧肌肉。然而，我们确实证明了我们的ENC的能力，有效地再生之间的天然神经的远端残端和修复网站在14的15只动物studied.Impact StatementVolumetric肌肉损失（VML）是一个临床相关的问题，目前的治疗方案是缺乏和组织工程骨骼肌提出了一个有前途的新的治疗选择。然而，制造临床相关尺寸的组织对于将该技术推进到临床是必要的。本研究的目的是评估我们扩大的组织工程化骨骼肌治疗VML在大型动物（绵羊）模型中6个月恢复后的疗效。
Tissue-engineered skeletal muscle is a promising novel therapy for the treatment of volumetric muscle loss (VML). Our laboratory has developed tissue-engineered skeletal muscle units (SMUs) and engineered neural conduits (ENCs), and modularly scaled them to clinically relevant sizes for the treatment of VML in a large animal (sheep) model. In a previous study, we evaluated the effects of the SMUs and ENCs in treating a 30% VML injury in the ovine peroneus tertius muscle after a 3-month recovery period. The goal of the current study was to expand on our 3-month study and evaluate the SMUs and ENCs in restoring muscle function after a 6-month recovery period. Six months after implantation, we found that the repair groups with the SMU (VML+SMU and VML+SMU+ENC) restored muscle mass to a level that was statistically indistinguishable from the uninjured contralateral muscle. In contrast, the muscle mass in the VML-Only group was significantly less than groups repaired with an SMU. Following the 6-month recovery from VML, the maximum tetanic force was significantly lower for all VML injured groups compared with the uninjured contralateral muscle. However, we did demonstrate the ability of our ENCs to effectively regenerate nerve between the distal stump of the native nerve and the repair site in 14 of the 15 animals studied.Impact StatementVolumetric muscle loss (VML) is a clinically relevant problem for which current treatment options are lacking and for which tissue-engineered skeletal muscle presents a promising novel therapeutic option. However, the fabrication of tissues of clinically relevant sizes is necessary for advancement of the technology to the clinic. This study aimed to evaluate the efficacy of our scaled-up tissue-engineered skeletal muscle to treat VML in a large animal (sheep) model after a 6-month recovery.
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影响因子：
4.1
作者：
Kim, John T.;Kasukonis, Benjamin;Wolchok, Jeffrey C.
通讯作者：
Wolchok, Jeffrey C.
影响因子：
4.1
作者：
VanDusen, Keith W.;Syverud, Brian C.;Larkin, Lisa M.
通讯作者：
Larkin, Lisa M.
影响因子：
20.3
作者：
Courey, Anthony J.;Horowitz, Jeffrey C.;Sisson, Thomas H.
通讯作者：
Sisson, Thomas H.
肺泡蛋白sis。
DOI：
--
发表时间：
2006
期刊：
Encycropedia of Respiratory Medicine (in press)
影响因子：
--
作者：
Choi;Murohara et al.;Seymour JF
通讯作者：
Seymour JF
影响因子：
9.7
作者：
Grasman JM;Zayas MJ;Page RL;Pins GD
通讯作者：
Pins GD