Epidermal Growth Factor-mediated Caveolin Recruitment to Early Endosomes and MAPK Activation
Epidermal Growth Factor-mediated Caveolin Recruitment to Early Endosomes and MAPK Activation
复制标题
表皮生长因子介导的 Caveolin 募集到早期内体和 MAPK 激活
DOI：
--
复制
发表时间：
2000
影响因子：
4.8
通讯作者：
C. Enrich
中科院分区：
文献类型：
--
作者：
A. Pol;Albert Lu;M. Pons;S. Peiró;C. Enrich
真核细胞的内吞区是一种复杂的细胞内结构，涉及多种内化分子的分类、加工和降解。近年来，通过小泡的摄取已成为另一种内化途径，似乎与一些信号转导途径直接相关。然而，调节小窝蛋白从细胞表面进入内吞区运输的机制、分子和结构在很大程度上是未知的。在这项研究中，使用正常的静止成纤维细胞（正常大鼠肾（NRK））来证明表皮生长因子导致小窝蛋白从细胞表面部分重新分布到细胞蛋白早期内吞室。用细胞松弛素D或拉runculin A处理NRK细胞可抑制该途径以及Mek和有丝分裂活化蛋白（MAP）激酶的伴随活化；然而，如果用filipin预处理细胞，细胞松弛素D不抑制表皮生长因子诱导的MAP激酶磷酸化。从这些结果我们得出结论，在NRK细胞中，完整的肌动蛋白细胞骨架是egf介导的小窝蛋白从细胞表面转运到早期内吞室和激活MAP激酶途径所必需的。
The endocytic compartment of eukaryotic cells is a complex intracellular structure involved in sorting, processing, and degradation of a great variety of internalized molecules. Recently, the uptake through caveolae has emerged as an alternative internalization pathway, which seems to be directly related with some signal transduction pathways. However, the mechanisms, molecules, and structures regulating the transport of caveolin from the cell surface into the endocytic compartment are largely unknown. In this study, normal quiescent fibroblasts (normal rat kidney (NRK)) were used to demonstrate that epidermal growth factor causes partial redistribution of caveolin from the cell surface into a cellubrevin early endocytic compartment. Treatment of NRK cells with cytochalasin D or latrunculin A inhibits this pathway and the concomitant activation of Mek and mitotic-activated protein (MAP) kinase; however, if cells were pre-treated with filipin, cytochalasin D does not inhibit the phosphorylation of MAP kinase induced by epidermal growth factor. From these results we conclude that in NRK cells the intact actin cytoskeleton is necessary for the EGF-mediated transport of caveolin from the cell surface into the early endocytic compartment and the activation of MAP kinase pathway.
登录
查看更多内容
DOI：
10.1073/pnas.92.22.10104
发表时间：
1995-10-24
影响因子：
11.1
作者：
SMART, EJ;YING, YS;ANDERSON, RGW
通讯作者：
ANDERSON, RGW
DOI：
10.1152/ajpcell.1997.272.3.c937
发表时间：
1997-03-01
影响因子：
5.5
作者：
Brown, LM;Fox, HL;Lynch, CJ
通讯作者：
Lynch, CJ
激活的 EGF 受体与包被的凹坑适应素的相互作用。
DOI：
10.1126/science.8342026
发表时间：
1993
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Sorkin,A;Carpenter,G
通讯作者：
Carpenter,G
钙调蛋白拮抗剂 W-13 可改变 Madin-Darby 犬肾细胞中的转胞吞作用、再循环和内吞途径的形态。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Apodaca,G;Enrich,C;Mostov,KE
通讯作者：
Mostov,KE