Development of interglobular dentine in rat molars and its relation to maturation of enamel
Development of interglobular dentine in rat molars and its relation to maturation of enamel
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大鼠磨牙球间牙本质的发育及其与牙釉质成熟的关系
DOI：
10.1007/s004290050115
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
H. Mayanagi
中科院分区：
文献类型：
--
作者：
M. Kagayama;Jing;Y. Sasano;Hidenori Sato;H. Mayanagi
应用N-乙酰-D-氨基葡萄糖凝集素（succinyl wheat germ agglutinin，sWGA）对3、7、14、21、42日龄Wistar大鼠第一上、下磨牙球间牙本质的发育进行了组织化学观察。sWGA染色成牙本质细胞的球间牙本质、前牙本质和高尔基区。第3天大鼠的第一磨牙中未形成球间牙本质，而第7天大鼠的第一磨牙中在无釉区附近出现球间牙本质。在14天的大鼠中，除了颈部区域外，冠状牙本质的大部分区域都存在球间牙本质。在牙本质和前牙本质之间的界面处，看到许多sWGA阴性的钙球，这表明球间牙本质在那里活跃地形成。在21天的大鼠，小叶间牙本质比14天的大鼠更多。球间牙本质存在于颈根牙本质以及冠状牙本质，包括颈部区域。42天组与21天组牙本质球间区分布相似，但sWGA结合荧光强度较低。由于球间牙本质的发育具有一定的时间和位置特异性，本文分析了球间牙本质的发育与成釉细胞发育阶段的关系。造模3天大鼠磨牙牙尖形成较薄的釉基质，造模7天大鼠牙尖釉基质厚度增加。这些牙齿中的成釉细胞处于分化或分泌阶段。分泌型成釉细胞、中间层细胞和釉基质的高尔基区和Tomes'突起呈弱阳性。无釉质区的上皮细胞也用sWGA染色。14日龄大鼠上颌第一磨牙成釉细胞除颈区处于过渡期外，其余均处于成熟期。sWGA染色的远缘和高尔基区的成熟成釉细胞以及乳头层的细胞。成熟期成釉细胞的远侧缘或厚或薄，呈皱褶端和光滑端。21日龄大鼠的第一磨牙中无成釉细胞，釉质的颈部与42日龄大鼠相似，被复层上皮覆盖。目前的研究表明，球间牙本质含有sWGA结合糖复合物，球间牙本质的形成在很大程度上与釉质成熟有关。这些结果表明，基质细胞的相互作用是重要的球间牙本质的发展。
Abstract The development of interglobular dentine in the first upper and lower molars of Wistar rats aged 3, 7, 14, 21, 42 days was examined histochemically using a lectin, succinyl wheat germ agglutinin (sWGA), which is specific for N-acetyl-D-glucosamine. sWGA stained the interglobular dentine, predentin and Golgi area of odontoblasts. Interglobular dentine was not formed in the first molars of 3-day rats, but appeared in those of 7-day rats near the enamel-free area. In 14-day rats, interglobular dentine was present in most areas of the coronal dentine except the cervical area. At the interface between dentine and predentin, numerous sWGA-negative calcospherites were seen, suggesting that the interglobular dentine is formed actively there. In 21-day rats, the interlobular dentine was more numerous than in 14-day rats. Interglobular dentine was present in the cervical root dentine as well as in the coronal dentine, including the cervical area. The distribution of interglobular dentine in 42-day rats was similar to that in 21-day rats, but fluorescence of sWGA binding was less intense in the former. Because the development of interglobular dentine appeared to be time and position specific its relation to the stages of ameloblasts was analysed. Thin enamel matrix was formed at cusps in molars of 3-day rats and thickness of enamel matrix increased in 7-day rats. In these teeth, the ameloblasts were at the differentiating or secretory stage. The Golgi area and Tomes’ processes of the secretory ameloblasts, the cells of intermediate layer and the enamel matrix were weakly positive with sWGA. The epithelial cells at the enamel-free area were also stained with sWGA. In 14-day rats, most of the ameloblasts in the first maxillary molars were at the maturative stage except in the cervical area, where the ameloblasts were at the transitional stage. sWGA stained the distal border and the Golgi area of the maturative ameloblasts as well as the cells of the papillary layer. The distal border of the maturative ameloblasts appeared either thick or thin, suggesting a ruffle-end and smooth-end of the cells. Ameloblasts were absent in the first molars of 21-day rats and the cervical part of the enamel was covered with the stratified epithelium like that of 42-day rats. The present study has demonstrated that interglobular dentine contains sWGA-binding glycoconjugates and the formation of the interglobular dentine is largely associated with the enamel maturation. These results suggest that matrix-to-cell interaction is important for the development of interglobular dentine.
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DOI：
10.1016/s0021-9258(18)98510-8
发表时间：
1991-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. Deutsch;Aron Palmon;Larry W. Fisherll;N. Kolodny;John D. Terminell;Marian F. Youngll
通讯作者：
D. Deutsch;Aron Palmon;Larry W. Fisherll;N. Kolodny;John D. Terminell;Marian F. Youngll
DOI：
10.1387/ijdb.7626423
发表时间：
1995
期刊：
The International journal of developmental biology
影响因子：
--
作者：
M. Zeichner‐David;Thomas GH Diekwisch;A. Fincham;Eduardo C. Lau;M. Macdougall;Janet Moradian-oldak;J. P. Simmer;Malcolm L. Snead;Harold C. Slavkin
通讯作者：
M. Zeichner‐David;Thomas GH Diekwisch;A. Fincham;Eduardo C. Lau;M. Macdougall;Janet Moradian-oldak;J. P. Simmer;Malcolm L. Snead;Harold C. Slavkin
在小鼠磨牙咬合“无牙釉质区域”发育过程中，内牙釉质上皮合成并分泌牙釉质蛋白。
DOI：
--
发表时间：
1991
期刊：
Journal of craniofacial genetics and developmental biology
影响因子：
--
作者：
Nakamura,M;BringasJr,P;Slavkin,HC
通讯作者：
Slavkin,HC
DOI：
10.1387/ijdb.7626404
发表时间：
1995-02
期刊：
The International journal of developmental biology
影响因子：
--
作者：
W. Butler;H. Ritchie
通讯作者：
W. Butler;H. Ritchie
小鼠磨牙器官发生的胎儿、新生儿和出生后早期阶段多种牙釉质蛋白的顺序表达和差异功能。
DOI：
10.1111/j.1432-0436.1988.tb00793.x
发表时间：
1988
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Slavkin,HC;Bessem,C;BringasJr,P;Zeichner-David,M;Nanci,A;Snead,ML
通讯作者：
Snead,ML