Upregulation of myelin and lymphocyte protein (MAL) after traumatic spinal cord injury in rats
Upregulation of myelin and lymphocyte protein (MAL) after traumatic spinal cord injury in rats
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大鼠脊髓创伤后髓磷脂和淋巴细胞蛋白（MAL）的上调
DOI：
10.1007/s10735-012-9469-0
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发表时间：
2013-04
影响因子：
3.2
通讯作者：
Gu, Haiyan
中科院分区：
文献类型：
--
作者：
Cui, Zhiming;Cui, Zhiming;Shen, Aiguo;Li, Weidong;Xu, Guanhua;Bao, Guofeng;Sun, Yuyu;Wang, Lingling;Gu, Haiyan
髓磷脂和淋巴细胞蛋白（MAL）被鉴定为一种四跨蛋白脂质，作为致密髓磷脂的组成部分，在少突胶质细胞和雪旺细胞中高度表达。据报道，它也是一种肿瘤抑制因子，并通过 Fas 途径诱导细胞凋亡。然而，其在脊髓损伤中的表达和功能仍不清楚，特别是在灰质中。在本研究中，我们建立了成年大鼠脊髓挫伤（SCI）模型，并检测了脊髓中MAL表达的动态变化。蛋白质印迹和免疫组织化学分析显示，MAL 存在于正常脊髓的灰质和白质中。它逐渐增加，在第 1 天达到峰值，然后在脊髓损伤后下降到基础水平。双重免疫荧光染色显示，在神经元和少突胶质细胞中发现了 MAL 免疫反应性。有趣的是，MAL 表达主要在神经元而不是少突胶质细胞中增加。我们还检查了活性 caspase-3 的表达谱，其变化与 MAL 的表达谱相关。此外，还检测到 MAL 与活性 caspase-3 的共定位。总之，这是首次描述脊髓损伤后灰质中 MAL 表达的变化。我们的结果提示 MAL 可能参与 SCI 后的 CNS 病理生理学。
Myelin and lymphocyte protein (MAL) was identified as a tetraspan proteolipid that is highly expressed by oligodendrocytes and Schwann cells as a component of compact myelin. It has also been reported to be a tumour suppressor and induces apoptosis through the Fas pathway. However, its expression and function in spinal cord injury are still unclear, especially in gray matter. In this study, we performed a spinal cord contusion injury (SCI) model in adult rats and detected the dynamic changes of MAL expression in spinal cord. Western blot and immunohistochemistry analysis revealed that MAL was present in gray and white matter of normal spinal cord. It gradually increased, got a peak at 1 day, and then declined to basal levels after spinal cord injury. Double immunofluorescence staining showed that MAL immunoreactivity was found in neurons and oligodendrocytes. Interestingly, MAL expression was increased predominantly in neurons rather than oligodendrocytes. We also examined the expression profiles of active caspase-3, whose changes were correlated with the expression profiles of MAL. Moreover, co-localization of MAL with active caspase-3 was detected. In conclusion, this is the first description of MAL expression changes in gray matter after spinal cord injury. Our results prompted that MAL might participate in CNS pathophysiology after SCI.
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影响因子：
3.7
作者：
Yin X;Yin Y;Cao FL;Chen YF;Peng Y;Hou WG;Sun SK;Luo ZJ
通讯作者：
Luo ZJ
影响因子：
6.1
作者：
Xuan Li;Xiao-Ling Zhang;Gang Shen;G. Tang
通讯作者：
Xuan Li;Xiao-Ling Zhang;Gang Shen;G. Tang
影响因子：
4.8
作者：
Puertollano, R;Li, SW;Alonso, MA
通讯作者：
Alonso, MA
影响因子：
2.9
作者：
Song, Myoung-Sub;Seo, Heung-Sik;Moon, Chanjong
通讯作者：
Moon, Chanjong
影响因子：
3.5
作者：
Gang Wu;Jianhua Cao;Chen Peng;Huiguang Yang;Zhiming Cui;Jian Zhao;Qiyun Wu;Jian-zhong Han
通讯作者：
Gang Wu;Jianhua Cao;Chen Peng;Huiguang Yang;Zhiming Cui;Jian Zhao;Qiyun Wu;Jian-zhong Han