Successive Site Translocating Inoculation Improved T Cell Responses Elicited by a DNA Vaccine Encoding SARS-CoV-2 S Protein.
Successive Site Translocating Inoculation Improved T Cell Responses Elicited by a DNA Vaccine Encoding SARS-CoV-2 S Protein.
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DOI：
10.3389/fimmu.2022.875236
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发表时间：
2022
影响因子：
7.3
通讯作者：
Wang, Wanhai
中科院分区：
文献类型：
--
作者：
Tian, Xiangxiang;Zhang, Yifan;He, Zhangyufan;Li, Shaoshuai;Yan, Dongmei;Zhu, Zhaoqin;Wan, Yanmin;Wang, Wanhai
关键词：
A variety of methods have been explored to increase delivery efficiencies for DNA vaccine. However, the immunogenicity of DNA vaccines has not been satisfactorily improved. Unlike most of the previous attempts, we provided evidence suggesting that changing the injection site successively (successively site-translocated inoculation, SSTI) could significantly enhance the immunogenicity of DNA vaccines in a previous study. To simplify the strategy and to evaluate its impact on candidate SARS-CoV-2 vaccines, we immunized mice with either a SARS-CoV-2 spike-based DNA vaccine or a spike protein subunit vaccine via three different inoculation strategies. Our data demonstrated that S protein specific antibody responses elicited by the DNA vaccine or the protein subunit vaccine showed no significant difference among different inoculation strategies. Of interest, compared with the conventional site fixed inoculation (SFI), both successive site-translocating inoculation (SSTI) and the simplified translocating inoculation (STI) strategy improved specific T cell responses elicited by the DNA vaccine. More specifically, the SSTI strategy significantly improved both the monofunctional (IFN-γ+IL-2-TNF-α-CD8+) and the multifunctional (IFN-γ+IL-2-TNF-α+CD8+, IFN-γ+IL-2-TNF-α+CD4+, IFN-γ+IL-2+TNF-α+CD4+) T cell responses, while the simplified translocating inoculation (STI) strategy significantly improved the multifunctional CD8+ (IFN-γ+IL-2-TNF-α+CD8+, IFN-γ+IL-2+TNF-α+CD8+) and CD4+ (IFN-γ+IL-2-TNF-α+CD4+, IFN-γ+IL-2+TNF-α+CD4+) T cell responses. The current study confirmed that changing the site of intra muscular injection can significantly improve the immunogenicity of DNA vaccines.
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影响因子：
16.6
作者：
Kundu R;Narean JS;Wang L;Fenn J;Pillay T;Fernandez ND;Conibear E;Koycheva A;Davies M;Tolosa-Wright M;Hakki S;Varro R;McDermott E;Hammett S;Cutajar J;Thwaites RS;Parker E;Rosadas C;McClure M;Tedder R;Taylor GP;Dunning J;Lalvani A
通讯作者：
Lalvani A
影响因子：
4.6
作者：
Ren Y;Wang N;Hu W;Zhang X;Xu J;Wan Y
通讯作者：
Wan Y
DNA疫苗的电穿孔输送：成功的前景。
影响因子：
7
作者：
Sardesai NY;Weiner DB
通讯作者：
Weiner DB
BNT162B2 mRNA COVID-19疫苗的安全性和功效。
DOI：
10.1056/nejmoa2034577
发表时间：
2020-12-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Polack FP;Thomas SJ;Kitchin N;Absalon J;Gurtman A;Lockhart S;Perez JL;Pérez Marc G;Moreira ED;Zerbini C;Bailey R;Swanson KA;Roychoudhury S;Koury K;Li P;Kalina WV;Cooper D;Frenck RW Jr;Hammitt LL;Türeci Ö;Nell H;Schaefer A;Ünal S;Tresnan DB;Mather S;Dormitzer PR;Şahin U;Jansen KU;Gruber WC;C4591001 Clinical Trial Group
通讯作者：
C4591001 Clinical Trial Group
影响因子：
7.8
作者：
Park JH;Lee HK
通讯作者：
Lee HK