The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation.
The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation.
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DOI：
10.1038/srep02866
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发表时间：
2013-10-09
影响因子：
4.6
通讯作者：
Shirasu, Ken
中科院分区：
文献类型：
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作者：
Lyons, Rebecca;Iwase, Akira;Gansewig, Thomas;Sherstnev, Alexander;Duc, Celine;Barton, Geoffrey J.;Hanada, Kousuke;Higuchi-Takeuchi, Mieko;Matsui, Minami;Sugimoto, Keiko;Kazan, Kemal;Simpson, Gordon G.;Shirasu, Ken
rna结合蛋白（rbp）在植物宿主与微生物相互作用中起着重要作用。在这项研究中，我们发现调节3 '端mRNA聚腺苷化的植物RBP FPA负调控拟南芥对细菌病原体丁香假单胞菌的基础抗性。一项定制芯片分析显示，flg22是一种源自细菌鞭毛蛋白的肽，可以诱导编码防御相关转录抑制因子乙烯反应因子4 （ERF4）的mRNA的选择性聚腺苷化异构体的表达，而ERF4是由FPA调节的。Flg22诱导缺乏erf相关的两亲性抑制（EAR）基序的ERF4新异构体的表达，而FPA抑制这种诱导。ERF4缺乏ear的异构体在体内作为转录激活因子，抑制flg22依赖性活性氧的爆发。我们建议FPA控制ERF4近端聚腺苷化位点的使用，这在数量上限制了防御反应的输出。
RNA-binding proteins (RBPs) play an important role in plant host-microbe interactions. In this study, we show that the plant RBP known as FPA, which regulates 3′-end mRNA polyadenylation, negatively regulates basal resistance to bacterial pathogen Pseudomonas syringae in Arabidopsis. A custom microarray analysis reveals that flg22, a peptide derived from bacterial flagellins, induces expression of alternatively polyadenylated isoforms of mRNA encoding the defence-related transcriptional repressor ETHYLENE RESPONSE FACTOR 4 (ERF4), which is regulated by FPA. Flg22 induces expression of a novel isoform of ERF4 that lacks the ERF-associated amphiphilic repression (EAR) motif, while FPA inhibits this induction. The EAR-lacking isoform of ERF4 acts as a transcriptional activator in vivo and suppresses the flg22-dependent reactive oxygen species burst. We propose that FPA controls use of proximal polyadenylation sites of ERF4, which quantitatively limit the defence response output.
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DOI：
10.1111/j.1365-313x.2007.03359.x
发表时间：
2008-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jin JB;Jin YH;Lee J;Miura K;Yoo CY;Kim WY;Van Oosten M;Hyun Y;Somers DE;Lee I;Yun DJ;Bressan RA;Hasegawa PM
通讯作者：
Hasegawa PM
影响因子：
11.8
作者：
Hornyik, Csaba;Terzi, Lionel C.;Simpson, Gordon G.
通讯作者：
Simpson, Gordon G.
影响因子：
7.4
作者：
FUJIWARA, T;HIRAI, MY;NAITO, S
通讯作者：
NAITO, S
影响因子：
64.8
作者：
Fu, Zheng Qing;Guo, Ming;Alfano, James R.
通讯作者：
Alfano, James R.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF