Local synthesis of natriuretic peptides in the eel intestine.
Local synthesis of natriuretic peptides in the eel intestine.
复制标题
鳗鱼肠道内局部合成利尿钠肽。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Y. Takei
中科院分区：
文献类型：
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作者：
C. Loretz;C. Pollina;H. Kaiya;H. Sakaguchi;Y. Takei
肠是广盐性鱼类在海洋中生存的主要肠壁调节器官，利钠肽参与了肠壁转运的调节。在鳗鲡肠中发现心房利钠肽（ANP）和心室利钠肽（VNP）。采用放射免疫分析法测定了ANP和VNP在高效液相色谱（HPLC）中的洗脱曲线。HPLC中免疫反应性ANP和VNP的洗脱时间与真实肽标准品的洗脱时间相同，并且与这些肽的相对分子质量一致。用荧光免疫组织化学方法对ANP和VNP进行组织定位。免疫反应细胞可见于肠前、中、后区上皮。从肠和随后的聚合酶链反应扩增分离的mRNA的逆转录产生适当大小的产品与ANP和VNP表达一致。总之，这些数据表明，利钠肽是在鳗鱼肠道局部合成的，而不是从循环中捕获的。
The intestine is a major osmoregulatory organ in euryhaline fishes which allows them to survive in the sea, and natriuretic peptides have been implicated in regulation of transmural transport. Atrial (ANP) and ventricular natriuretic peptide (VNP) were identified in eel intestine. Elution profiles of ANP and VNP from high-performance liquid chromatography (HPLC) were determined by radioimmunoassay using highly specific antisera. Elution times of immunoreactive ANP and VNP in HPLC were identical to those of authentic peptide standards and were consistent with the relative molecular masses of these peptides. Tissue localization of ANP and VNP was accomplished by fluorescence immunohistochemistry. Immunoreactive cells were observed in the epithelium of anterior, middle, and posterior regions of intestine. Reverse transcription of mRNA isolated from intestine and subsequent polymerase chain reaction amplification yielded appropriate-size products consistent with ANP and VNP expression. Together, these data show that natriuretic peptides are synthesized locally in eel intestine, rather than trapped from the circulation.
影响因子：
3.1
作者：
GowerJr,WR;Dietz,JR;Vesely,DL;Finley,CL;Skolnick,KA;Fabri,PJ;Cooper,DR;Chalfant,CE
通讯作者：
Chalfant,CE