Antioxidant activity and anti-proliferative effect of a bioactive peptide from chickpea (Cicer arietinum L.)
Antioxidant activity and anti-proliferative effect of a bioactive peptide from chickpea (Cicer arietinum L.)
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鹰嘴豆 (Cicer arietinum L.) 生物活性肽的抗氧化活性和抗增殖作用
DOI：
10.1016/j.foodres.2015.09.027
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发表时间：
2015-11
影响因子：
8.1
通讯作者：
Xiaohong Kou
中科院分区：
文献类型：
--
作者：
Zhaohui Xue;Haichao Wen;Lijuan Zhai;Yanqing Yu;Yanni Li;Wancong Yu;Aiqing Cheng;Cen Wang;Xiaohong Kou
我们之前的研究发现了一种源自鹰嘴豆白蛋白水解物的鹰嘴豆肽（CPe-III），具有高抗氧化活性。通过化学合成对应于CPe-III的氨基酸序列(RQSHFANAQP)的CPe-III-S肽以确定其生物活性。还原力、羟自由基、DPPH自由基、ABTS自由基清除试验和DCFH-DA试验均表现出显着的剂量依赖性活性。细胞活力测定表明对 MCF-7 和 MDA-MB-231 细胞具有高抑制活性，EC50 值为 2.38 和 1.50 μmol/mL。 ELISA 试剂盒检测 p53 蛋白随着 CPe-III-S 浓度的增加而增加。通过分子对接确定肽与p53蛋白的特异性结合。这些结果表明CPe-III-S通过增加p53有效抑制乳腺癌细胞的增殖。这是基于鹰嘴豆酶解产物CPe-III的合成肽的首次报道，具有抗氧化活性和抗增殖作用。
Our previous study identified a chickpea peptide (CPe-III) derived from chickpea albumin hydrolysate which exhibited a high antioxidant activity. CPe-III-S peptide corresponding to amino acid sequences of CPe-III (RQSHFANAQP) was synthesized chemically to determine the bioactive activity. The reducing power, hydroxyl radical, DPPH radical, ABTS radical scavenging assay and DCFH-DA assay showed significant dose-dependent activities. The cell viability assay indicated a high inhibition activity on MCF-7 and MDA-MB-231 cells with EC50values of 2.38 and 1.50 μmol/mL. The p53 protein increased with increasing concentration of CPe-III-S tested by ELISA Kit. The specific binding of peptide and p53 protein was determined by means of molecular docking. These results indicated that CPe-III-S effectively inhibited the proliferation of breast cancer cells by increasing the p53. This is the first report of the synthetic peptide which based on CPe-III derived from the enzymatic hydrolysate of chickpea to exhibit the antioxidant activity and antiproliferative effect.
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影响因子：
3
作者：
C.V.S. Siva Prasad;Saurabh Gupta;A. Gaponenko;M. Tiwari
通讯作者：
C.V.S. Siva Prasad;Saurabh Gupta;A. Gaponenko;M. Tiwari
影响因子：
14.9
作者：
Mashiach E;Nussinov R;Wolfson HJ
通讯作者：
Wolfson HJ
影响因子：
64.8
作者：
K.J. Harrington;P. Nenclares
通讯作者：
K.J. Harrington;P. Nenclares
DOI：
10.1002/9780470110546.ch2
发表时间：
2006-10
期刊：
Methods of biochemical analysis
影响因子：
--
作者：
B. Halliwell;M. Grootveld;J. Gutteridge
通讯作者：
B. Halliwell;M. Grootveld;J. Gutteridge
影响因子：
13.8
作者：
J. Meldolesi
通讯作者：
J. Meldolesi