Analysis of p16 gene deletion and point mutation in breast carcinoma.
Analysis of p16 gene deletion and point mutation in breast carcinoma.
复制标题
乳腺癌p16基因缺失和点突变分析。
作者：
Bruno Quesnel;P. Fenaux;Nathalie Philippe;J. Fournier;Jacques Bonneterre;Claude Preudhomme;J. Peyrat
我们分别从35例和33例乳腺癌的新鲜肿瘤样本中，通过Southern分析和单链构象多态性（SSCP）分析寻找p16基因缺失，通过DNA直接测序寻找p16基因点突变。未发现p16基因纯合子缺失。p16基因错义点突变仅在1例患者中发现。患者淋巴细胞中没有这种点突变，排除了多态性或种系突变的可能性。这些发现表明p16基因的改变在乳腺癌中很少观察到。
We looked for p16 gene deletion by Southern analysis and p16 gene point mutation by single-stranded conformation polymorphism (SSCP) analysis and direct sequencing of DNA from fresh tumour samples of 35 and 33 breast carcinomas respectively. No homozygous p16 gene deletion was found in any case. A missense point mutation of the p16 gene was found in only one patient. This point mutation was absent from the patient's lymphocytes, ruling out a polymorphism or a germline mutation. These findings suggest that p16 gene alterations are rarely observed in breast carcinoma.
人类乳腺癌中 CDKN2 (MTS1/p16ink4) 的突变分析。
影响因子：
11.2
作者：
Xu,L;Sgroi,D;Sterner,CJ;Beauchamp,RL;Pinney,DM;Keel,S;Ueki,K;Rutter,JL;Buckler,AJ;Louis,DN
通讯作者：
Louis,DN
影响因子：
56.9
作者：
KAMB, A;GRUIS, NA;SKOLNICK, MH
通讯作者：
SKOLNICK, MH