EPO improved neurologic outcome in rat pups late after traumatic brain injury.
EPO improved neurologic outcome in rat pups late after traumatic brain injury.
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DOI：
10.1016/j.braindev.2018.01.003
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发表时间：
2018-05
影响因子：
1.7
通讯作者：
Rodesch CK
中科院分区：
文献类型：
--
作者：
Schober ME;Requena DF;Rodesch CK
在成年大鼠中，促红细胞生成素改善创伤性脑损伤后早期和晚期的结果，与脑源性神经营养因子水平的增加相关。使用我们的儿童创伤性脑损伤模型，在17天大的大鼠中控制皮质撞击，我们先前表明促红细胞生成素在损伤后的前两天增加海马神经元分数。促红细胞生成素还降低了半胱天冬酶3（一种由脑源性神经营养因子调节的凋亡酶）的激活，并改善了损伤后14天的新物体识别测试。关于促红细胞生成素对发育性创伤性脑损伤后脑源性神经营养因子表达、组织学和认知功能的长期影响的数据缺乏。我们假设，促红细胞生成素将增加脑源性神经营养因子，并改善长期的对象识别后，大鼠幼崽控制皮质的影响，与海马神经元分数增加。研究方法：幼鼠在损伤或假手术后1、24和48小时和7天接受促红细胞生成素或载体，随后在35天进行组织学检查，在成年时进行新物体识别测试，并在损伤后早期和晚期测量脑源性神经营养因子。结果：促红细胞生成素提高了新物体识别的性能，并保留海马体积，但不是神经元分数，损伤后的后期。结论：促红细胞生成素治疗大鼠的物体识别改善与创伤性脑损伤后晚期海马体积的保留相关。促红细胞生成素被批准用于治疗各种儿科疾病。再加上令人兴奋的实验和临床研究表明，它是有益的新生儿缺氧缺血性脑损伤后，我们的初步研究结果支持进一步研究促红细胞生成素的使用后，发育性创伤性脑损伤。
In adult rats, erythropoietin improved outcomes early and late after traumatic brain injury, associated with increased levels of Brain Derived Neurotrophic Factor. Using our model of pediatric traumatic brain injury, controlled cortical impact in 17-day old rats, we previously showed that erythropoietin increased hippocampal neuronal fraction in the first two days after injury. Erythropoietin also decreased activation of caspase3, an apoptotic enzyme modulated by Brain Derived Neurotrophic Factor, and improved Novel Object Recognition testing 14 days after injury. Data on long-term effects of erythropoietin on Brain Derived Neurotrophic Factor expression, histology and cognitive function after developmental traumatic brain injury are lacking. We hypothesized that erythropoietin would increase Brain Derived Neurotrophic Factor and improve long-term object recognition in rat pups after controlled cortical impact, associated with increased neuronal fraction in the hippocampus. Methods: Rats pups received erythropoietin or vehicle at 1, 24, and 48 hours and 7 days after injury or sham surgery followed by histology at 35 days, Novel Object Recognition testing at adulthood, and Brain Derived Neurotrophic Factor measurements early and late after injury. Results: Erythropoietin improved Novel Object Recognition performance and preserved hippocampal volume, but not neuronal fraction, late after injury. Conclusions: Improved object recognition in erythropoietin treated rats was associated with preserved hippocampal volume late after traumatic brain injury. Erythropoietin is approved to treat various pediatric conditions. Coupled with exciting experimental and clinical studies suggesting it is beneficial after neonatal hypoxic ischemic brain injury, our preliminary findings support further study of erythropoietin use after developmental traumatic brain injury.
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2.1
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通讯作者：
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作者：
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