Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling.
Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling.
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DOI：
10.1186/1471-2229-10-97
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发表时间：
2010-05-26
影响因子：
5.3
通讯作者：
Arimura G
中科院分区：
文献类型：
--
作者：
Kanchiswamy CN;Takahashi H;Quadro S;Maffei ME;Bossi S;Bertea C;Zebelo SA;Muroi A;Ishihama N;Yoshioka H;Boland W;Takabayashi J;Endo Y;Sawasaki T;Arimura G
植物Ca ~（2+）信号参与了害虫入侵后的多种细胞内信号转导途径。Ca 2+依赖性蛋白激酶（CPKs）等Ca 2+结合感觉蛋白被认为是介导昆虫取食后Ca 2+内流的信号转导途径。然而，到目前为止，这一预测还无法验证。为了研究CPK在草食动物应答信号通路中的作用，我们筛选了被食源性通吃草食动物Spodoptera littoralis损伤的拟南芥CPK突变体的特征。在昆虫攻击后，cpk 3和cpk 13突变体与野生型植物相比显示出较低的植物防御素基因PDF1.2的转录水平。CPK级联反应与由防御相关的植物激素如茉莉酸和乙烯介导的草食诱导的信号传导途径没有直接联系。CPK 3还被认为参与了植食性和创伤性损伤后细胞内Ca ~（2+）水平的负反馈调节。CPK 3蛋白与一系列底物的体外激酶测定表明，该蛋白在Ca 2+存在下磷酸化转录因子（包括ERF 1、HsfB 2a和CZF 1/ZFAR 1）。CPK 13仅强烈磷酸化HsfB 2a，而不考虑Ca 2+的存在。此外，在体内农杆菌渗透试验表明，CPK 3或CPK 13衍生的磷酸化的热休克因子（HsfB 2a）促进PDF1.2转录激活的防御反应。这些结果揭示了两种拟南芥CPK（CPK 3和CPK 13）通过HsfB 2a介导的防御相关转录机制的调节参与了草食诱导的信号网络。该级联不参与植物激素相关的信号通路，而是直接影响防御反应的转录因子。
Plant Ca2+ signals are involved in a wide array of intracellular signaling pathways after pest invasion. Ca2+-binding sensory proteins such as Ca2+-dependent protein kinases (CPKs) have been predicted to mediate the signaling following Ca2+ influx after insect herbivory. However, until now this prediction was not testable. To investigate the roles CPKs play in a herbivore response-signaling pathway, we screened the characteristics of Arabidopsis CPK mutants damaged by a feeding generalist herbivore, Spodoptera littoralis. Following insect attack, the cpk3 and cpk13 mutants showed lower transcript levels of plant defensin gene PDF1.2 compared to wild-type plants. The CPK cascade was not directly linked to the herbivory-induced signaling pathways that were mediated by defense-related phytohormones such as jasmonic acid and ethylene. CPK3 was also suggested to be involved in a negative feedback regulation of the cytosolic Ca2+ levels after herbivory and wounding damage. In vitro kinase assays of CPK3 protein with a suite of substrates demonstrated that the protein phosphorylates transcription factors (including ERF1, HsfB2a and CZF1/ZFAR1) in the presence of Ca2+. CPK13 strongly phosphorylated only HsfB2a, irrespective of the presence of Ca2+. Furthermore, in vivo agroinfiltration assays showed that CPK3-or CPK13-derived phosphorylation of a heat shock factor (HsfB2a) promotes PDF1.2 transcriptional activation in the defense response. These results reveal the involvement of two Arabidopsis CPKs (CPK3 and CPK13) in the herbivory-induced signaling network via HsfB2a-mediated regulation of the defense-related transcriptional machinery. This cascade is not involved in the phytohormone-related signaling pathways, but rather directly impacts transcription factors for defense responses.
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DOI：
10.1073/pnas.0510664103
发表时间：
2006-02-07
影响因子：
11.1
作者：
Gallagher, E;Gao, M;Karin, M
通讯作者：
Karin, M
DOI：
10.1073/pnas.0502954102
发表时间：
2005-07-26
影响因子：
11.1
作者：
Ludwig, AA;Saitoh, H;Romeis, T
通讯作者：
Romeis, T
影响因子：
6.9
作者：
Ludwig, AA;Romeis, T;Jones, JDG
通讯作者：
Jones, JDG
影响因子：
7.4
作者：
Chaudhuri, S;Seal, A;DasGupta, M
通讯作者：
DasGupta, M
影响因子：
7.4
作者：
Dammann, C;Ichida, A;Harper, JF
通讯作者：
Harper, JF