Molecular identification and codon optimization analysis of major virulence encoding genes of Aeromonas hydrophila
Molecular identification and codon optimization analysis of major virulence encoding genes of Aeromonas hydrophila
复制标题
嗜水气单胞菌主要毒力编码基因的分子鉴定及密码子优化分析
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
N. Sood
中科院分区：
文献类型：
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作者：
Vijai Singh;D. Chaudhary;I. Mani;P. Somvanshi;G. Rathore;N. Sood
嗜水气单胞菌是水生和陆生动物以及人类的机会致病菌。本研究的目的是调查来自 40 条鱼肌肉和 12 个水样的 25 个嗜水放线杆菌分离株。所有分离株均在表型和基因型上筛选是否存在毒力因子，例如气溶素、溶血素和脂肪酶。所有分离株均产生脂肪酶，而只有 60% 的分离株与红细胞产生 b-溶血。 PCR检测证实了气溶素、溶血素和脂肪酶3个毒力基因的存在，保守片段PCR扩增呈阳性。这些还针对在大肠杆菌中过度表达进行了密码子优化，并与野生型 DNA 序列相比获得了高密码子适应指数。这些发现可能有助于更好地表达基因，以扩大候选疫苗的生产规模。
关键词：嗜水气单胞菌、毒力因子、PCR、密码子优化、疫苗。
Aeromonas hydrophila is an opportunistic pathogen of aquatic and terrestrial animals and human beings. The aim of this study is to investigate 25 isolates of A. hydrophilafrom 40 fish muscles and 12 water samples. All isolates are screened for the presence of virulence factors such as aerolysin, hemolysin and lipase both phenotypically as well as genotypically. All the isolates produced lipase, whereas only 60% of isolates produced b-hemolysis with RBCs. The presence of 3 virulence genes aerolysin, hemolysin and lipase were confirmed by PCR assay, which gave positive PCR amplification for conserved fragment. These were also codon optimized for over expression in Escherichia coli and obtained the high codon adaptation index in comparison to wild type of DNA sequences. These finding may help to better expression of gene for scale up production of vaccine candidates.
Keywords: Aeromonas hydrophila, virulence factor, PCR, codon optimization, vaccine.
影响因子：
14.9
作者：
SHARP, PM;LI, WH
通讯作者：
LI, WH