Quercetin glucosides promote ischemia-induced angiogenesis, but do not promote tumor growth.
Quercetin glucosides promote ischemia-induced angiogenesis, but do not promote tumor growth.
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槲皮素葡萄糖苷促进缺血诱导的血管生成，但不促进肿瘤生长。
DOI：
10.1016/j.lfs.2013.09.005
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sata M.
中科院分区：
文献类型：
--
作者：
Sumi M;Tateishi N;Shibata H;Ohki T;Sata M.
目的：膳食中黄酮类化合物的摄入量与冠心病死亡率、心肌梗死和中风的发生率呈显著负相关。槲皮素是我们饮食中最常见的黄酮类化合物之一，具有多种有利的生物活性。槲皮素糖苷是异槲皮苷的酶促转糖基化产物，与槲皮素相比，具有较高的水溶性和生物利用度。在这里，我们研究了槲皮素葡萄糖苷对小鼠后肢缺血模型中侧支发育的影响。主要方法我们通过切除右股动脉在24至32周龄的雄性C3 H/HeJ小鼠中诱导后肢缺血。然后，每天灌胃给予0.5%羧甲基纤维素（对照）或槲皮素葡糖苷（100 mg/kg/天）。每周用激光多普勒成像仪监测血流量。关键发现槲皮素葡萄糖苷能显著促进缺血腿的血流量恢复（4周时血流量比：对照组，0.57 ± 0.11;槲皮素葡萄糖苷，0.95 ± 0.10，p < 0.05）。此外，抗CD 31免疫染色显示槲皮素葡糖苷增加缺血肌肉中的毛细血管密度（对照，200 ± 24/mm 2;槲皮素葡糖苷，364 ± 41/mm 2，p< 0.01）。槲皮素葡萄糖苷没有促进肿瘤生长。槲皮素葡萄糖苷的有益作用在eNOS缺陷小鼠中被消除。显著性这些结果表明槲皮素葡萄糖苷可能具有促进缺血组织血管生成的治疗潜力。
AimsDietary flavonoid intake shows a significant inverse association with mortality from coronary heart disease, incidence of myocardial infarction and stroke. Quercetin is one of the most common flavonoids in our diet and has several favorable biological activities. Quercetin glucosides, which are enzymatically trans-glycosylated isoquercitrin, have high water-solubility and bioavailability compared with quercetin. Here, we investigated the effects of quercetin glucosides on collateral development in a murine hindlimb ischemia model.Main methodsWe induced hindlimb ischemia in 24- to 32-week-old male C3H/HeJ mice by resecting the right femoral artery. Then, 0.5% carboxymethyl cellulose (control) or quercetin glucosides (100 mg/kg/day) were administered daily by gavage. Blood flow was monitored weekly by laser Doppler imaging.Key findingsRecovery of blood flow to the ischemic leg was significantly enhanced by quercetin glucosides (blood flow ratio at 4 weeks: control, 0.57 ± 0.11; quercetin glucosides, 0.95 ± 0.10,p< 0.05). Furthermore, anti-CD31 immunostaining revealed that quercetin glucosides increased capillary density in the ischemic muscle (control, 200 ± 24/mm2; quercetin glucosides, 364 ± 41/mm2,p< 0.01). Quercetin glucosides did not promote tumor growth. The beneficial effect of quercetin glucosides was abrogated in eNOS-deficient mice.SignificanceThese results suggest that quercetin glucosides may have therapeutic potential to promote angiogenesis in ischemic tissue.
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影响因子：
8.3
作者：
Sata, M;Nishimatsu, H;Nagai, R
通讯作者：
Nagai, R
影响因子：
6.1
作者：
Daud, Noor Huda;Aung, Cho Sanda;Parat, Marie-Odile
通讯作者：
Parat, Marie-Odile
影响因子：
15.9
作者：
Murohara, T;Asahara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
8.3
作者：
Amano, K;Matsubara, H;Iwasaka, T
通讯作者：
Iwasaka, T
影响因子：
37.8
作者：
Couffinhal, T;Silver, M;Isner, JM
通讯作者：
Isner, JM