Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa
Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa
复制标题
铜绿假单胞菌 rpoN 基因的核苷酸序列和两个下游开放阅读框的表征
作者：
Shouguang Jin;Karyn S. Ishimoto;S. Lory
铜绿假单胞菌的rpoN基因是许多不同基因表达所必需的，从几类细菌粘附素到氨基酸生物合成酶。已确定rpoN基因及其侧翼区的核苷酸序列。推导的rpoN产物的氨基酸序列与其他微生物的RpoN蛋白序列高度同源。此外，两个开放阅读框架（ORF 1和ORF 2）编码肽的103和154个氨基酸长，分别被发现的rpoN基因的下游。这两个ORF产物与位于其它微生物中rpoN基因附近的类似ORF产物具有高度的氨基酸序列同源性。当在基本培养基上繁殖时，任一ORF中的突变导致铜绿假单胞菌世代时间的显著增加。这些突变对菌毛蛋白或鞭毛蛋白基因的表达没有影响，其表达依赖于RpoN。互补分析表明，这两个ORF位于同一个转录单位，两个ORF突变体在基本培养基上的生长缺陷是由于ORF 2的突变效应。ORF突变对铜绿假单胞菌在基本培养基中生长的不利影响可以通过添加谷氨酰胺而不是精氨酸、谷氨酸、组氨酸或脯氨酸来抑制。由于铜绿假单胞菌的rpoN突变体显示出生长所需的相同氨基酸，因此ORF2产物很可能作为一些但不是所有RpoN控制基因的共诱导物起作用。
The rpoN gene of Pseudomonas aeruginosa is required for the expression of a number of diverse genes, ranging from several classes of bacterial adhesins to enzymes for amino acid biosynthesis. The nucleotide sequence of the rpoN gene and its flanking region has been determined. The deduced amino acid sequence of the rpoN product is highly homologous to sequences of RpoN proteins of other microorganisms. Moreover, two open reading frames (ORF1 and ORF2) encoding peptides of 103 and 154 amino acids long, respectively, were found downstream of the rpoN gene. These two ORF products have a high degree of amino acid sequence homology with products of similar ORFs located adjacent to the rpoN genes in other microorganisms. Mutations in either ORF lead to a significant increase in P. aeruginosa generation time when propagated on minimal medium. These mutations had no effect on the expression of pilin or flagellin genes, whose expression depends on RpoN. Complementation analysis showed that the two ORFs are in the same transcriptional unit and the growth defects of the two ORF mutants on minimal medium are due to mutational effects on ORF2. The adverse effect of the ORF mutations on the growth of P. aeruginosa in minimal media can be suppressed by the addition of glutamine but not arginine, glutamate, histidine, or proline. Since rpoN mutants of P. aeruginosa display this same amino acid requirement for growth, the ORF2 product very likely functions as a coinducer of some but not all of the RpoN-controlled genes.
DOI：
10.1073/pnas.86.6.1954
发表时间：
1989-03-01
影响因子：
11.1
作者：
ISHIMOTO, KS;LORY, S
通讯作者：
LORY, S
DOI：
10.1073/pnas.81.19.6110
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MEYER, TF;BILLYARD, E;SO, M
通讯作者：
SO, M