Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout.
Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout.
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DOI：
10.1186/s12953-021-00180-0
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发表时间：
2021-10-11
期刊：
影响因子：
2
通讯作者：
Peng A
中科院分区：
文献类型：
--
作者：
Chen G;Cheng J;Yu H;Huang X;Bao H;Qin L;Wang L;Song Y;Liu X;Peng A
痛风是基于高尿酸血症的免疫反应性关节炎的一种常见而复杂的形式，而症状会转向缓解甚至恶化。因此，很难早期识别无症状高尿酸血症（AHU）患者是否容易发生急性痛风发作，也很难预测痛风从缓解到发作的过程。在这里，我们报道血浆蛋白谱可以区分急性痛风（AG），痛风缓解（RG）， AHU患者和健康对照。我们建立了一种基于相对和绝对定量等压标签（iTRAQ）和平行反应监测（PRM）的血浆蛋白检测方法，用于测定AG组（n = 8）、RG组（n = 7）、AHU组（n = 7）和健康对照组（n = 8）的血浆蛋白。鉴定出组蛋白H2A、H2B、血栓spontin -1 （THBS1）、髓过氧化物酶（MPO）、补体C2、补体组分C8 β链（C8B）、α -1-酸性糖蛋白1 （ORM1）、α -胰蛋白酶抑制剂重链H4 （ITIH4）、碳酸酐酶1 （CA1）、血清白蛋白（ALB）和多聚蛋白1 （MMRN1）等11种差异表达蛋白。Histone H2A、Histone H2B和THBS1可能是维持痛风过程平衡和稳定的最具影响力的调节因子。补体和凝血级联反应是痛风过程的主要功能途径之一。组蛋白H2A、H2B和THBS1是区分痛风发作与AHU或RG的新型生物标志物的潜在候选基因，为痛风过程的预后、治疗和管理提供了新的理论见解。这项研究不是临床试验。在线版本包含补充材料，可在10.1186/s12953-021-00180-0获得。
Gout is a common and complex form of immunoreactive arthritis based on hyperuricemia, while the symptoms would turn to remission or even got worse. So, it is hard to early identify whether an asymptomatic hyperuricemia (AHU) patient will be susceptible to get acute gout attack and it is also hard to predict the process of gout remission to flare. Here, we report that the plasma proteins profile can distinguish among acute gout (AG), remission of gout (RG), AHU patients, and healthy controls. We established an isobaric tags for relative and absolute quantification (iTRAQ) and parallel reaction monitoring (PRM) based method to measure the plasma proteins for AG group (n = 8), RG group (n = 7), AHU group (n = 7) and healthy controls (n = 8). Eleven differentially expressed proteins such as Histone H2A, Histone H2B, Thrombospondin-1 (THBS1), Myeloperoxidase (MPO), Complement C2, Complement component C8 beta chain (C8B), Alpha-1-acid glycoprotein 1 (ORM1), Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Carbonic anhydrase 1 (CA1), Serum albumin (ALB) and Multimerin-1 (MMRN1) were identified. Histone H2A, Histone H2B and THBS1 might be the strongest influential regulator to maintain the balance and stability of the gout process. The complement and coagulation cascades is one of the main functional pathways in the mechanism of gout process. Histone H2A, Histone H2B and THBS1 are potential candidate genes for novel biomarkers in discriminating gout attack from AHU or RG, providing new theoretical insights for the prognosis, treatment, and management of gout process. This study is not a clinical trial. The online version contains supplementary material available at 10.1186/s12953-021-00180-0.
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