Synaptic interactions between the terminals of slow‐adapting type II mechanoreceptor afferents and neurones expressing γ‐aminobutyric acid‐ and glycine‐like immunoreactivity in the rat spinal cord
Synaptic interactions between the terminals of slow‐adapting type II mechanoreceptor afferents and neurones expressing γ‐aminobutyric acid‐ and glycine‐like immunoreactivity in the rat spinal cord
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大鼠脊髓中慢适应 II 型机械感受器传入神经元与表达 γ-氨基丁酸和甘氨酸样免疫反应性的神经元之间的突触相互作用
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
A. Watson
中科院分区：
文献类型：
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作者：
A. Watson
本研究的目的是分析大鼠脊髓III-V板层慢适应II型(SAII)传入终末的突触相互作用。在注射神经生物素之前，通过细胞内记录和电子显微镜检查，观察SAII传入纤维的生理学特征。轴突末梢连续切片，用抗γ-氨基丁酸或甘氨酸的抗体进行免疫金标记法。计算机辅助重建显示不同类型突触在突触末端和传递端的相对分布。89%的Bouton接受了轴突突触接触，平均每Bouton接触次数为3.5次。59%的突触前轴突同时表达GABA和甘氨酸，45%的突触前突触仅表达GABA。95%的牙周有轴-树接触，平均接触树突数为1.6个。半数以上的突触后树突直径大于1μm。23%的人对甘氨酸呈免疫反应，71%的人对这两种抗体都不呈免疫反应。突触三联体中，突触前的轴突与突触后的树突相接触的突触三联体出现在63%的突触上。这些结果是根据对大鼠和猫的其他低阈值机械感受性终末的类似研究，并在已知的从缓慢和快速适应的机械感受器携带信息的感觉神经元间的背景下进行讨论的。J.Comp.神经。471：168-179,2004。©2004 Wiley-Liss Inc.
The object of this study was to analyse the synaptic interactions of slow‐adapting type II (SAII) afferent terminals in laminae III–V of the rat spinal cord. The axons of SAII afferents were physiologically characterized by intracellular recording before injection with neurobiotin and preparation for electron microscopy. Axon terminals were serially sectioned and immunolabelled with antibodies against γ‐aminobutyric acid (GABA) or glycine by using a postembedding immunogold procedure. Computer‐aided reconstruction was used to reveal the relative distribution of different types of synapses on terminal and en passant synaptic boutons. Eighty‐nine percent of boutons received axoaxonic synaptic contacts, the mean number of contacts per bouton being 3.5. Fifty‐nine percent of presynaptic axons were immunoreactive for both GABA and glycine and 45% for GABA alone. Most boutons (95%) made axodendritic contacts, and the mean number of dendrites contacted was 1.6. More than half of the postsynaptic dendrites were greater than 1 μm in diameter. Twenty‐three percent were immunoreactive for glycine, and 71% were not immunoreactive for either antibody. Synaptic triads in which an axon presynaptic to the afferent was also in contact with a dendrite postsynaptic to the afferent were seen at 63% of boutons. These results are discussed in the light of similar studies of other low‐threshold mechanoreceptive afferent terminals in the rat and cat and in the context of what is known of the sensory interneurones carrying information from slow‐ and rapid‐adapting mechanoreceptors. J. Comp. Neurol. 471:168–179, 2004. © 2004 Wiley‐Liss, Inc.
影响因子：
2.5
作者：
LEEM, JW;WILLIS, WD;CHUNG, JM
通讯作者：
CHUNG, JM
体外哺乳动物脊髓背角 III-V 层中间神经元的功能特性和轴突终止。
影响因子：
2.5
作者：
Schneider,SP
通讯作者：
Schneider,SP
影响因子：
2.5
作者：
LEEM, JW;WILLIS, WD;CHUNG, JM
通讯作者：
CHUNG, JM