Hyperthermophilic Aquifex aeolicus initiates primer synthesis on a limited set of trinucleotides comprised of cytosines and guanines.
Hyperthermophilic Aquifex aeolicus initiates primer synthesis on a limited set of trinucleotides comprised of cytosines and guanines.
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DOI：
10.1093/nar/gkn461
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发表时间：
2008-09
影响因子：
14.9
通讯作者：
Hinrichs SH
中科院分区：
文献类型：
--
作者：
Larson MA;Bressani R;Sayood K;Corn JE;Berger JM;Griep MA;Hinrichs SH
极端嗜热水蛭在细菌系统发育树上的位置引起了很大的争议。我们研究了在高温下生长的适应性是否会改变复制机制的关键功能成分，特别是DnaG引物酶。尽管细菌引物的结构是保守的，但据推测，风芽孢杆菌的三核苷酸起始特异性与其他微生物不同，是对地热环境的适应。为了确定风衣沙蚤引物酶的全范围活性，设计了包含所有可能的三核苷酸起始序列的两个寡核苷酸。其中一个筛选模板支持引物合成，并利用所得引物的长度预测可能的起始三核苷酸。三核苷酸特异性模板的使用表明，风衣沙蚕引物酶的首选起始三核苷酸序列为5 ‘ -d(CCC)-3 ’。另外两个序列5 ‘ -d(GCC)-3 ’和d(CGC)-3 '也支持起始，但程度要小得多。这些三核苷酸都不是已知的其他微生物引物所使用的识别序列。这些结果表明，风草引物酶的起始特异性可能代表了对嗜热环境的适应。
The placement of the extreme thermophile Aquifex aeolicus in the bacterial phylogenetic tree has evoked much controversy. We investigated whether adaptations for growth at high temperatures would alter a key functional component of the replication machinery, specifically DnaG primase. Although the structure of bacterial primases is conserved, the trinucleotide initiation specificity for A. aeolicus was hypothesized to differ from other microbes as an adaptation to a geothermal milieu. To determine the full range of A. aeolicus primase activity, two oligonucleotides were designed that comprised all potential trinucleotide initiation sequences. One of the screening templates supported primer synthesis and the lengths of the resulting primers were used to predict possible initiation trinucleotides. Use of trinucleotide-specific templates demonstrated that the preferred initiation trinucleotide sequence for A. aeolicus primase was 5′-d(CCC)-3′. Two other sequences, 5′-d(GCC)-3′ and d(CGC)-3′, were also capable of supporting initiation, but to a much lesser degree. None of these trinucleotides were known to be recognition sequences used by other microbial primases. These results suggest that the initiation specificity of A. aeolicus primase may represent an adaptation to a thermophilic environment.
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DOI：
10.1098/rspb.2000.1397
发表时间：
2001-03-07
影响因子：
4.7
作者：
Hurst, LD;Merchant, AR
通讯作者：
Merchant, AR
影响因子：
3.6
作者：
Koepsell, Scott A.;Larson, Marilynn A.;Griep, Mark A.
通讯作者：
Griep, Mark A.
影响因子：
3.4
作者：
Coenye, T;Vandamme, P
通讯作者：
Vandamme, P
影响因子：
3.2
作者：
GROMPE, M;VERSALOVIC, J;LUPSKI, JR
通讯作者：
LUPSKI, JR
影响因子：
4.8
作者：
Frick, DN;Richardson, CC
通讯作者：
Richardson, CC