MECHANISMS BEHIND THE RESISTANCE OF SPHEROIDS TO PHOTODYNAMIC TREATMENT: A FLOW CYTOMETRY STUDY
MECHANISMS BEHIND THE RESISTANCE OF SPHEROIDS TO PHOTODYNAMIC TREATMENT: A FLOW CYTOMETRY STUDY
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球体抵抗光动力处理背后的机制：流式细胞术研究
DOI：
10.1111/j.1751-1097.1992.tb04257.x
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发表时间：
1992
影响因子：
3.3
通讯作者：
J. Moore
中科院分区：
文献类型：
--
作者：
C. West;J. Moore
翻译后摘要-细胞异质性对光动力学治疗（PDT）的反应的影响进行了研究，使用人类结肠腺癌线WiDr，生长为球状体，并暴露于血卟啉衍生物。球体显示出对PDT的显著球体尺寸依赖性抗性。使用流式细胞仪，基于药物荧光，从由500 μm直径球状体制备的单细胞悬液中分离细胞亚群。低荧光的细胞已被证明对PDT具有抗性，具有较小的中值细胞体积，并且在G1型细胞中增强。这些细胞还显示低密度脂蛋白摄取减少。结果表明，球体大小依赖性的PDT抗性与随着球体大小的增加而降低的生长分数有关。药物摄取的异质性可能是临床PDT的潜在限制。
Abstract— The influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size‐dependent resistance to PDT. Using a flow cytometer, cell sub‐populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 μm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1‐type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size‐dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT.
单层、球状和裸鼠体内生长的人类结肠腺癌的辐射反应。
影响因子：
3.4
作者：
West,CM;Sutherland,RM
通讯作者：
Sutherland,RM
影响因子：
56.9
作者：
SUTHERLAND, RM
通讯作者：
SUTHERLAND, RM