Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation.
Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation.
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核心发酵（COFE）颗粒聚焦的糖酵解mRNA定位和转化为燃料葡萄糖发酵。
DOI：
10.1016/j.isci.2021.102069
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发表时间：
2021-02-19
期刊：
影响因子：
5.8
通讯作者：
Ashe MP
中科院分区：
文献类型：
--
作者：
Morales-Polanco F;Bates C;Lui J;Casson J;Solari CA;Pizzinga M;Forte G;Griffin C;Garner KEL;Burt HE;Dixon HL;Hubbard S;Portela P;Ashe MP
糖酵解是生物体内葡萄糖代谢的基本途径，糖酵解酶是细胞中最丰富的蛋白质之一。它们在这种水平上的表达带来了特别的挑战。在这里，我们证明了糖酵解mRNA定位于酵母和人类细胞中的颗粒。详细的活细胞和酵母中的smFISH研究表明，mRNA在颗粒中被积极翻译，这种翻译对于定位至关重要。此外，这种安排可能有助于糖酵解途径的更高水平的组织和控制。事实上，细胞所需的发酵程度与mRNA定位于颗粒的程度有内在联系。在此基础上，我们将这些颗粒称为核心发酵（CoFe）颗粒;它们似乎代表翻译工厂，允许高水平协调酶合成关键代谢途径。糖酵解mRNA共定位并在RNA颗粒中翻译糖酵解mRNA颗粒在细胞发酵时很重要糖酵解蛋白翻译可能在这些翻译工厂中协调因此，“CoFe颗粒”是糖酵解蛋白的核心发酵工厂分子生物学;细胞生物学
Glycolysis is a fundamental metabolic pathway for glucose catabolism across biology, and glycolytic enzymes are among the most abundant proteins in cells. Their expression at such levels provides a particular challenge. Here we demonstrate that the glycolytic mRNAs are localized to granules in yeast and human cells. Detailed live cell and smFISH studies in yeast show that the mRNAs are actively translated in granules, and this translation appears critical for the localization. Furthermore, this arrangement is likely to facilitate the higher level organization and control of the glycolytic pathway. Indeed, the degree of fermentation required by cells is intrinsically connected to the extent of mRNA localization to granules. On this basis, we term these granules, core fermentation (CoFe) granules; they appear to represent translation factories, allowing high-level coordinated enzyme synthesis for a critical metabolic pathway. Glycolytic mRNAs colocalize and are translated in RNA granules The glycolytic mRNA granules are important when cells are fermenting Glycolytic protein translation is likely coordinated in these translation factories Therefore, “CoFe granules” are core fermentation factories for glycolytic proteins Molecular biology; cell biology
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
DOI：
10.1016/j.tem.2015.09.012
发表时间：
2015-12
期刊：
Trends in endocrinology and metabolism: TEM
影响因子：
--
作者：
Castello A;Hentze MW;Preiss T
通讯作者：
Preiss T
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
5.4
作者：
Araiza-Olivera, Daniela;Chiquete-Felix, Natalia;Uribe-Carvajal, Salvador
通讯作者：
Uribe-Carvajal, Salvador
影响因子：
21.3
作者：
Benanti, Jennifer A.;Cheung, Stephanie K.;Toczyski, David P.
通讯作者：
Toczyski, David P.