Gap junction channel activity in short-term cultured human detrusor myocyte cell pairs: gating and unitary conductances.
Gap junction channel activity in short-term cultured human detrusor myocyte cell pairs: gating and unitary conductances.
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短期培养的人逼尿肌细胞对中的间隙连接通道活性：门控和单一电导。
DOI：
10.1152/ajpcell.00027.2006
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Christ,GeorgeJ
中科院分区：
文献类型：
--
作者：
Wang,H-Z;Brink,PeterR;Christ,GeorgeJ
一些独立的研究表明，通过缝隙连接的细胞间通讯调节了膀胱生理，而且，连接通讯的改变可能导致逼尿肌过度活动。然而，据我们所知，目前还没有人或大鼠逼尿肌细胞间缝隙连接介导的细胞间电流的直接记录。用Northern和Western印迹法检测冰冻的人膀胱组织和短期培养的人逼尿肌细胞中连接蛋白的表达。双全细胞贴片(DWCP)记录显示，人逼尿肌细胞对连接良好，平均连接电导为6.5±4.6 ns(0.1~15 ns，n=22)。人逼尿肌细胞宏观缝隙连接通道电流表现出与同型连接蛋白43类似的电压依赖性。归一化跨结电导-电压(GJ-VJ)关系是对称的，符合两态玻尔兹曼关系(Gmin≈0.33，V0=63.6 mV，Z=0.117或等于2.95门控电荷)，提示存在双向电压门控机制。在对称165mmCSCL中，测得的单通道斜率电导为∼120ps(全开通道)和∼26ps(主次状态)。偶尔，还观察到其他亚导状态。单通道平均开放时间随Vj的增大而减小，说明宏观结电流随Vj的减小而减小。在新鲜分离的大鼠逼尿肌细胞的DWCP中观察到了定性相似的电生理特征。这些数据证实和扩展了先前的观察，并与在其他类型的平滑肌细胞中发现的Cx43介导的细胞间通讯的报告一致。
Several independent lines of investigation indicate that intercellular communication through gap junctions modulates bladder physiology and, moreover, that altered junctional communication may contribute to detrusor overactivity. However, as far as we are aware, there are still no direct recordings of gap junction-mediated intercellular currents between human or rat detrusor myocytes. Northern and Western blots were used to identify connexin expression in frozen human bladder tissue and short-term cultured human detrusor myocytes. Double whole cell patch (DWCP) recording revealed that human detrusor myocyte cell pairs were well coupled with an average junctional conductance of 6.5 ± 4.6 nS (ranging from 0.1 to 15 nS,n= 22 cell pairs). Macroscopic gap junction channel currents in human detrusor myocytes exhibited voltage dependence similar to homotypic connexin43. The normalized transjunctional conductance-voltage (Gj-Vj) relationship was symmetrical and well described by a two-state Boltzmann relation (Gmin≈ 0.33,V0= 63.6 mV,Z= 0.117 or equal to 2.95 gating charges), suggestive of a bilateral voltage-gated mechanism. In symmetric 165 mM CsCl, the measured single-channel slope conductance was ∼120 pS for the fully open channel and ∼26 pS for the major substate. Occasionally, other subconductance states were also observed. The single-channel mean open time declined with increasingVj, accounting for theVj-dependent decline of macroscopic junctional current. Qualitatively similar electrophysiological characteristics were observed in DWCP of freshly isolated rat detrusor myocytes. These data confirm and extend previous observations and are consistent with reports in other smooth muscle cells types in which Cx43-mediated intercellular communication has been identified.
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影响因子：
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作者：
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DOI：
10.1152/ajpheart.1995.269.4.h1481
发表时间：
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期刊：
The American journal of physiology.
影响因子：
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作者：
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作者：
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