Requirement for CCNB1 in mouse spermatogenesis.
Requirement for CCNB1 in mouse spermatogenesis.
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CCNB1 在小鼠精子发生中的需求
DOI：
10.1038/cddis.2017.555
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发表时间：
2017-10-26
影响因子：
9
通讯作者：
Liu YX
中科院分区：
文献类型：
--
作者：
Tang JX;Li J;Cheng JM;Hu B;Sun TC;Li XY;Batool A;Wang ZP;Wang XX;Deng SL;Zhang Y;Chen SR;Huang X;Liu YX
精子发生是一个高度复杂、协调有序的过程，涉及到雄性生殖细胞的有丝分裂和减数分裂。细胞周期蛋白B1（CCNB 1）是细胞周期的重要调控因子，在小鼠胚胎发育过程中起重要作用。然而，CCNB 1在哺乳动物精子发生中的作用仍不清楚。在这里，我们使用雄性生殖细胞中缺乏CCNB 1的条件性基因敲除小鼠测试了对CCNB 1的需求。我们发现，CCNB 1在生殖母细胞和精原细胞中的缺失导致小鼠雄性生殖细胞耗竭而导致不育。无CCNB 1的生殖细胞和精原细胞不能正常增殖，凋亡增加。此外，CCNB 1在精原细胞中的消融可能通过下调Lin 28 a和上调let-7 miRNA来促进其分化。然而，在减数分裂前雄性生殖细胞中去除CCNB 1对精母细胞减数分裂和雄性育性没有影响，这表明CCNB 1可能与精母细胞减数分裂有关。总的来说，这些结果表明，CCNB 1是至关重要的生殖细胞和精原细胞的增殖，但可能是多余的精母细胞减数分裂在小鼠精子发生。
Spermatogenesis, which involves mitosis and meiosis of male germ cells, is a highly complicated and coordinately ordered process. Cyclin B1 (CCNB1), an important regulator in cell cycle machinery, is proved essential for mouse embryonic development. However, the role of CCNB1 in mammalian spermatogenesis remains unclear. Here we tested the requirement for CCNB1 using conditional knockout mice lacking CCNB1 in male germ cells. We found that ablation of CCNB1 in gonocytes and spermatogonia led to mouse sterile caused by the male germ cells’ depletion. Gonocyte and spermatogonia without CCNB1 is unable to proliferate normally and apoptosis increased. Moreover, CCNB1 ablation in spermatogonia may promote their differentiation by downregulating Lin28a and upregulating let-7 miRNA. However, ablation of CCNB1 in premeiotic male germ cells did not have an effect on meiosis of spermatocytes and male fertility, suggesting that CCNB1 may be dispensable for meiosis of spermatocytes. Collectively, these results indicate that CCNB1 is critically required for the proliferation of gonocytes and spermatogonia but may be redundant in meiosis of spermatocytes in mouse spermatogenesis.
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影响因子：
1.5
作者：
Gallardo, Teresa;Shirley, Lane;Castrillon, Diego H.
通讯作者：
Castrillon, Diego H.
DOI：
10.1073/pnas.1115201109
发表时间：
2012-03-06
影响因子：
11.1
作者：
Diril, M. Kasim;Ratnacaram, Chandrahas Koumar;Kaldis, Philipp
通讯作者：
Kaldis, Philipp
影响因子：
64.8
作者：
GAUTIER, J;MATSUKAWA, T;MALLER, J
通讯作者：
MALLER, J
影响因子：
5.3
作者：
Filipponi, Doria;Hobbs, Robin M.;Dolci, Susanna
通讯作者：
Dolci, Susanna
DOI：
10.1083/jcb.98.4.1247
发表时间：
1984-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gerhart J;Wu M;Kirschner M
通讯作者：
Kirschner M