Cultured peripheral blood mast cells from chronic idiopathic urticaria patients spontaneously degranulate upon IgE sensitization: Relationship to expression of Syk and SHIP-2.
Cultured peripheral blood mast cells from chronic idiopathic urticaria patients spontaneously degranulate upon IgE sensitization: Relationship to expression of Syk and SHIP-2.
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DOI：
10.1016/j.clim.2009.05.003
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Vonakis BM
中科院分区：
文献类型：
--
作者：
Saini SS;Paterniti M;Vasagar K;Gibbons SP Jr;Sterba PM;Vonakis BM
最近，在慢性特发性荨麻疹（CIU）受试者的嗜碱性粒细胞中发现了涉及肌醇脂质磷酸酶的FcεRI通路的信号变化。根据嗜碱性粒细胞Fcε RI介导的组胺脱粒特征，我们将CIU受试者分为两组，CIU应答者（CIU R）或CIU无应答者（CIU NR）。在本研究中，我们比较了SHIP-1，SHIP-2和Syk蛋白的表达，组胺释放（HR）从肥大细胞（MC）培养的外周血CIU R，CIU NR，和正常人。与CIU NR（Syk：p=0.038：SHIP-2：p= 0.038）和正常人（Syk：p=0.042：SHIP-2：p =0.027）相比，CIU R供体的MC含有显著增加的Syk和减少的SHIP-2。与正常人相比，CIU供体的自发HR增加了两倍（p=0.04）。总之，我们的研究结果表明，荨麻疹性MC可能倾向于在IgE致敏后自发地脱髓鞘，从而导致与CIU相关的皮炎增加。
Recently, signaling changes in the FcεRI pathway involving inositol lipid phosphatases have been identified in the basophils of chronic idiopathic urticaria (CIU) subjects. Based on the profile of basophil FcεRI-mediated histamine degranulation, we have segregated CIU subjects into two groups, CIU Responder (CIU R) or CIU Nonresponder (CIU NR). In the present study, we compared expression of SHIP-1, SHIP-2, and Syk protein to histamine release (HR) from mast cells (MC) cultured from the peripheral blood of CIU R, CIU NR, and normal subjects. The MC of CIU R donors contained significantly increased Syk and decreased SHIP-2 as compared to CIU NR (Syk: p=0.038: SHIP-2: p=0.038) and normals (Syk: p=0.042: SHIP-2: p=0.027). Spontaneous HR from CIU donors was increased two-fold compared to normals (p=0.04). In summary, our results suggest a possible predilection for urticarial MC to spontaneously degranulate upon IgE sensitization contributing to the increased pruritis associated with CIU.
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影响因子：
24.3
作者：
Cruse, G;Kaur, D;Bradding, P
通讯作者：
Bradding, P
影响因子：
158.5
作者：
HIDE, M;FRANCIS, DM;GREAVES, MW
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影响因子：
14.2
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DOI：
10.1124/jpet.106.109058
发表时间：
2006-12-01
影响因子：
3.5
作者：
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通讯作者：
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影响因子：
14.2
作者：
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通讯作者：
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