A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence
A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence
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编码含有 DUF266 的蛋白质的 OsPLS3 基因中的 22 bp 缺失与水稻叶片衰老有关
DOI：
10.1007/s11103-018-0758-1
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发表时间：
2018-08
影响因子：
5.1
通讯作者：
Pan Gang
中科院分区：
文献类型：
--
作者：
Li Kunyu;Chen Yaodong;Luo Yanmin;Huang Fudeng;Zhao Chaoyue;Cheng Fangmin;Xiang Xun;Pan Gang
关键信息TheOsPLS3基因座是通过基于图谱的克隆获得的，该基因编码一个含有DUF266的蛋白质。OsPLS3调控水稻叶片衰老的开始。糖基转移酶(GTS)是植物次生代谢物修饰所需的最重要的酶群之一，在植物生长发育中起着至关重要的作用，但大多数GTS的生物学功能尚不清楚。本文报道了一个新的水稻叶片早衰突变体(Ospls3)的鉴定和鉴定。通过基于图谱的克隆策略，我们确定了OsPLS3基因编码未知功能域266(DUF266)蛋白的22bp缺失是导致ospls3突变体叶片早衰表型的基础。随着野生型水稻叶片衰老，OsPLS3基因的表达水平逐渐下降，表明OsPLS3在调控叶片衰老中起着负性作用。生理分析、组织化学染色和透射电子显微镜分析表明，ospls3突变体比野生型积累了更高水平的乙烯和活性氧。此外，ospls3突变体对外源1-氨基环丙烷-1-羧酸、H_2O_2和高水平的细胞分裂素表现出超敏反应。我们的结果表明，含有DUF266的基因OsPLS3在水稻叶片衰老的开始过程中发挥了重要作用，部分是通过细胞分裂素和乙烯信号转导。
Key messageTheOsPLS3locus was isolated by map-based cloning that encodes a DUF266-containing protein. OsPLS3regulates the onset of leaf senescence in rice. Glycosyltransferases (GTs) are one of the most important enzyme groups required for the modification of plant secondary metabolites and play a crucial role in plant growth and development, however the biological functions of most GTs remain elusive. We reported here the identification and characterization of a novel Oryza sativa premature leaf senescence mutant (ospls3). Through map-based cloning strategy, we determined that 22-bp deletion in the OsPLS3 gene encoding a domain of unknown function 266 (DUF266)-containing protein, a member of GT14-like, underlies the premature leaf senescence phenotype in the ospls3 mutant. The OsPLS3 mRNA levels progressively declined with the age-dependent leaf senescence in wild-type rice, implying a negative role of OsPLS3 in regulating leaf senescence. Physiological analysis, and histochemical staining and transmission electron microscopy assays indicated that the ospls3 mutant accumulated higher levels of ethylene and reactive oxygen species than its wild type. Furthermore, the ospls3 mutant showed hypersensitivity to exogenous 1-aminocyclopropane-1-carboxylic acid, H2O2 and high level of cytokinins. Our results indicated that the DUF266-containing gene OsPLS3 plays an important role in the onset of leaf senescence, in part through cytokinin and ethylene signaling in rice.
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对乙烯生物合成中蛋白质更新调节的新见解。
影响因子：
3.8
作者：
Yoon GM
通讯作者：
Yoon GM
DOI：
--
发表时间：
1971
期刊：
--
影响因子：
--
作者：
S. Yoshida;D. Forno;J. Cock
通讯作者：
S. Yoshida;D. Forno;J. Cock
影响因子：
7.4
作者：
KAO, CH;YANG, SF
通讯作者：
YANG, SF
DOI：
10.1073/pnas.76.1.170
发表时间：
1979-01-01
影响因子：
11.1
作者：
ADAMS, DO;YANG, SF
通讯作者：
YANG, SF
影响因子：
7.2
作者：
GRBIC, V;BLEECKER, AB
通讯作者：
BLEECKER, AB