Detection of calcifications in vivo and ex vivo after brain injury in rat using SWIFT.
Detection of calcifications in vivo and ex vivo after brain injury in rat using SWIFT.
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DOI：
10.1016/j.neuroimage.2012.03.002
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发表时间：
2012-07-16
期刊：
影响因子：
5.7
通讯作者：
Grohn, Olli
中科院分区：
文献类型：
--
作者：
Lehto, Lauri Juhani;Sierra, Alejandra;Corum, Curtis Andrew;Zhang, Jinjin;Idiyatullin, Djaudat;Pitkanen, Asla;Garwood, Michael;Grohn, Olli
钙化是许多脑部疾病的病理学组成部分之一。使用MRI，它们通常通过利用幅度图像中的负对比度来检测。钙化比组织更具抗磁性，导致磁场干扰，可以在相位MR图像中看到。大多数相位成像研究使用基于梯度回波的脉冲序列。在这里，SWIFT的相位分量，一个几乎为零的采集延迟序列，被用来检测钙化离体和在癫痫持续状态和创伤性脑损伤的大鼠模型体内。钙化检测相位和想象SWIFT图像的基础上，他们的偶极子一样的磁场干扰。在幅值SWIFT图像中，钙化被区分为低信号和高信号。低信号钙化显示大的结晶颗粒，周围炎性细胞较少，而高信号钙化包含小颗粒，炎性细胞较多。SWIFT幅度图像中的钙化大小与茜素染色组织切片中的钙化大小相关。我们的数据表明，SWIFT是可能更好地保存信号附近的钙化或其他领域的干扰相比，梯度回波，由于其短的采集延迟和宽的激励带宽。此外，还定量描述了SWIFT脉冲序列在偶极磁场附近的相位衬度不均匀性。钙化的体内检测提供了一种工具，以探测神经退行性疾病的病理进展。特别是，它似乎为脑损伤后钙化周围的炎性细胞提供了替代标记。
Calcifications represent one component of pathology in many brain diseases. With MRI, they are most often detected by exploiting negative contrast in magnitude images. Calcifications are more diamagnetic than tissue, leading to a magnetic field disturbance that can be seen in phase MR images. Most phase imaging studies use gradient recalled echo based pulse sequences. Here, the phase component of SWIFT, a virtually zero acquisition delay sequence, was used to detect calcifications ex vivo and in vivo in rat models of status epilepticus and traumatic brain injury. Calcifications were detected in phase and imaginary SWIFT images based on their dipole like magnetic field disturbances. In magnitude SWIFT images, calcifications were distinguished as hypointense and hyperintense. Hypointense calcifications showed large crystallized granules with few surrounding inflammatory cells, while hyperintense calcifications contained small granules with the presence of more inflammatory cells. The size of the calcifications in SWIFT magnitude images correlated with that in Alizarin stained histological sections. Our data indicate that SWIFT is likely to better preserve signal in the proximity of a calcification or other field perturber in comparison to gradient echo due to its short acquisition delay and broad excitation bandwidth. Furthermore, a quantitative description for the phase contrast near dipole magnetic field inhomogeneities for the SWIFT pulse sequence is given. In vivo detection of calcifications provides a tool to probe the progression of pathology in neurodegenerative diseases. In particular, it appears to provide a surrogate marker for inflammatory cells around the calcifications after brain injury.
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影响因子：
2.2
作者：
Idiyatullin, Djaudat;Corum, Curt;Garwood, Michael
通讯作者：
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影响因子：
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