Characterization, Expression Profiling, and Biochemical Analyses of the Cinnamoyl-CoA Reductase Gene Family for Lignin Synthesis in Alfalfa Plants.
Characterization, Expression Profiling, and Biochemical Analyses of the Cinnamoyl-CoA Reductase Gene Family for Lignin Synthesis in Alfalfa Plants.
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苜蓿植物中用于木质素合成的肉桂酰辅酶 A 还原酶基因家族的表征、表达谱和生化分析
DOI：
10.3390/ijms23147762
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发表时间：
2022-07-14
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
肉桂酰辅酶A还原酶（CCR）是植物木质素合成的关键酶，在植物次生细胞壁发育和环境胁迫防御中发挥作用。苜蓿是一种主要的豆科饲料，品质优良，但木质素含量会对饲料消化率产生负面影响。目前，有关苜蓿 CCR 特征、基因表达及其在木质素代谢中的作用的信息有限。在这项研究中，我们鉴定了苜蓿 CCR 基因家族的 30 个成员。此外，基因结构、保守基序和进化分析表明，MsCCR1-7 可能具有 CCR 功能，而 23 个 MsCCR 类似物分为三类。 MsCCRs/MsCCR-likes 的表达模式表明它们在植物发育、环境胁迫响应和植物激素处理中的作用。这些结果与其启动子中的顺式元件一致。组织化学染色显示，随着发育，木质素积累逐渐加深，这与基因表达结果一致。此外，还纯化了重组MsCCR1和MsCCR-like1，并在四种底物下测试了动力学参数。此外，MsCCR1和MsCCR-like1蛋白的三维结构模型显示了底物结合基序H212(X)2K215R263的差异。这些结果对于未来豆类饲料品质改良和生物燃料工业工程的进一步应用具有重要意义。
Cinnamoyl-CoA reductase (CCR) is a pivotal enzyme in plant lignin synthesis, which has a role in plant secondary cell wall development and environmental stress defense. Alfalfa is a predominant legume forage with excellent quality, but the lignin content negatively affects fodder digestibility. Currently, there is limited information on CCR characteristics, gene expression, and its role in lignin metabolism in alfalfa. In this study, we identified 30 members in the CCR gene family of Medicago sativa. In addition, gene structure, conserved motif, and evolution analysis suggested MsCCR1–7 presumably functioned as CCR, while the 23 MsCCR-likes fell into three categories. The expression patterns of MsCCRs/MsCCR-likes suggested their role in plant development, response to environmental stresses, and phytohormone treatment. These results were consistent with the cis-elements in their promoters. Histochemical staining showed that lignin accumulation gradually deepened with the development, which was consistent with gene expression results. Furthermore, recombinant MsCCR1 and MsCCR-like1 were purified and the kinetic parameters were tested under four substrates. In addition, three-dimensional structure models of MsCCR1 and MsCCR-like1 proteins showed the difference in the substrate-binding motif H212(X)2K215R263. These results will be useful for further application for legume forage quality modification and biofuels industry engineering in the future.
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影响因子：
9.4
作者：
Carocha, Victor;Soler, Marcal;Grima-Pettenati, Jacqueline
通讯作者：
Grima-Pettenati, Jacqueline
DOI：
10.1073/pnas.1523264113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Beckers, Bram;De Beeck, Michiel Op;Vangronsveld, Jaco
通讯作者：
Vangronsveld, Jaco
毛白杨肉桂酰辅酶A还原酶（CCR）基因家族的表征揭示了CCR的酶活性位点和进化
影响因子：
4.3
作者：
Chao, Nan;Li, Ning;Gai, Ying
通讯作者：
Gai, Ying
影响因子：
46.9
作者：
Chen, Fang;Dixon, Richard A.
通讯作者：
Dixon, Richard A.
影响因子：
5.6
作者：
Chakraborty, Animikha;Viswanath, Aswini;Malipatil, Renuka;Semalaiyappan, Janani;Shah, Priya;Ronanki, Swarna;Rathore, Abhishek;Singh, Sumer Pal;Govindaraj, Mahalingam;Tonapi, Vilas A.;Thirunavukkarasu, Nepolean
通讯作者：
Thirunavukkarasu, Nepolean