Identification of Candidate Genes Regulating Drought Tolerance in Pearl Millet.
Identification of Candidate Genes Regulating Drought Tolerance in Pearl Millet.
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DOI：
10.3390/ijms23136907
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发表时间：
2022-06-21
影响因子：
5.6
通讯作者：
Thirunavukkarasu, Nepolean
中科院分区：
文献类型：
--
作者：
Chakraborty, Animikha;Viswanath, Aswini;Malipatil, Renuka;Semalaiyappan, Janani;Shah, Priya;Ronanki, Swarna;Rathore, Abhishek;Singh, Sumer Pal;Govindaraj, Mahalingam;Tonapi, Vilas A.;Thirunavukkarasu, Nepolean
珍珠粟是干旱和半干旱生态系统中维持粮食和饲料生产的重要作物。植物对干旱胁迫的耐受性吸引了我们通过功能基因组学的方法来研究其细胞和分子机制，以提高谷物产量。在这里，我们研究了48个自交系代表四个不同成熟度组在开花期的干旱反应。将74个干旱响应基因分为阿坝信号转导、激素信号转导、离子和渗透平衡、TF调节、分子适应、信号转导、生理适应、解毒等8个功能群，并对其进行了全面的研究。在干旱响应基因的保守基序中，蛋白激酶和MYB结构域蛋白是最保守的。对谷子作物中干旱基因的计算机比较分析表明，谷子与珍珠粟有大多数直系同源物。在谷子作物中鉴定的698种单倍型中，MyC 2和Myb 4具有最大的单倍型。蛋白质-蛋白质相互作用网络将ABI 2、P5 CS、CDPK、DREB、MYB和CYP 707 A3确定为主要枢纽基因。表达分析表明，存在共同的，以及独特的干旱响应基因的成熟组。根据基因表达谱鉴定了各成熟期组的耐旱基因型。在几个基因家族中，阿坝信号，转铁蛋白和信号蛋白是未来的贡献者耐旱性成熟组。功能验证的基因可用作珍珠粟和其他粟作物的回交育种、基因组选择和基因编辑方案的有希望的候选基因，以提高易干旱的干旱和半干旱生态系统的产量。
Pearl millet is an important crop of the arid and semi-arid ecologies to sustain food and fodder production. The greater tolerance to drought stress attracts us to examine its cellular and molecular mechanisms via functional genomics approaches to augment the grain yield. Here, we studied the drought response of 48 inbreds representing four different maturity groups at the flowering stage. A set of 74 drought-responsive genes were separated into five major phylogenic groups belonging to eight functional groups, namely ABA signaling, hormone signaling, ion and osmotic homeostasis, TF-mediated regulation, molecular adaptation, signal transduction, physiological adaptation, detoxification, which were comprehensively studied. Among the conserved motifs of the drought-responsive genes, the protein kinases and MYB domain proteins were the most conserved ones. Comparative in-silico analysis of the drought genes across millet crops showed foxtail millet had most orthologs with pearl millet. Of 698 haplotypes identified across millet crops, MyC2 and Myb4 had maximum haplotypes. The protein–protein interaction network identified ABI2, P5CS, CDPK, DREB, MYB, and CYP707A3 as major hub genes. The expression assay showed the presence of common as well as unique drought-responsive genes across maturity groups. Drought tolerant genotypes in respective maturity groups were identified from the expression pattern of genes. Among several gene families, ABA signaling, TFs, and signaling proteins were the prospective contributors to drought tolerance across maturity groups. The functionally validated genes could be used as promising candidates in backcross breeding, genomic selection, and gene-editing schemes in pearl millet and other millet crops to increase the yield in drought-prone arid and semi-arid ecologies.
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影响因子：
5.9
作者：
Bhat JA;Yu D;Bohra A;Ganie SA;Varshney RK
通讯作者：
Varshney RK
影响因子：
3.5
作者：
Buitrago-Bitar MA;Cortés AJ;López-Hernández F;Londoño-Caicedo JM;Muñoz-Florez JE;Muñoz LC;Blair MW
通讯作者：
Blair MW
分子生态学和在野生和培养的共同豆类中与干旱相关的ASR基因多态性中的选择（叶状豆类叶）。
影响因子：
2.9
作者：
Cortés AJ;Chavarro MC;Madriñán S;This D;Blair MW
通讯作者：
Blair MW
影响因子：
3.5
作者：
Cortés AJ;López-Hernández F
通讯作者：
López-Hernández F
DOI：
10.1093/bioinformatics/btv433
发表时间：
2015-11-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Cheong WH;Tan YC;Yap SJ;Ng KP
通讯作者：
Ng KP