A balancing act in transcription regulation by response regulators: titration of transcription factor activity by decoy DNA binding sites.
A balancing act in transcription regulation by response regulators: titration of transcription factor activity by decoy DNA binding sites.
复制标题
DOI：
10.1093/nar/gkab935
复制
发表时间：
2021-11-18
影响因子：
14.9
通讯作者：
Stock AM
中科院分区：
文献类型：
--
作者：
Gao R;Helfant LJ;Wu T;Li Z;Brokaw SE;Stock AM
Studies of transcription regulation are often focused on binding of transcription factors (TFs) to a small number of promoters of interest. It is often assumed that TFs are in great excess to their binding sites (TFBSs) and competition for TFs between DNA sites is seldom considered. With increasing evidence that TFBSs are exceedingly abundant for many TFs and significant variations in TF and TFBS numbers occur during growth, the interplay between a TF and all TFBSs should not be ignored. Here, we use additional decoy DNA sites to quantitatively analyze how the relative abundance of a TF to its TFBSs impacts the steady-state level and onset time of gene expression for the auto-activated Escherichia coli PhoB response regulator. We show that increasing numbers of decoy sites progressively delayed transcription activation and lowered promoter activities. Perturbation of transcription regulation by additional TFBSs did not require extreme numbers of decoys, suggesting that PhoB is approximately at capacity for its DNA sites. Addition of decoys also converted a graded response to a bi-modal response. We developed a binding competition model that captures the major features of experimental observations, providing a quantitative framework to assess how variations in TFs and TFBSs influence transcriptional responses.
登录
查看更多内容
影响因子：
6.4
作者：
Gao R;Stock AM
通讯作者：
Stock AM
影响因子：
7.7
作者：
Ali MZ;Parisutham V;Choubey S;Brewster RC
通讯作者：
Brewster RC
影响因子：
2.4
作者：
Das, Swetamber;Choubey, Sandeep
通讯作者：
Choubey, Sandeep
影响因子：
64.8
作者：
Dekel, E;Alon, U
通讯作者：
Alon, U
影响因子：
4.5
作者：
Gao R;Stock AM
通讯作者：
Stock AM