Imprinting analysis of porcine DIO3 gene in two fetal stages and association analysis with carcass and meat quality traits
Imprinting analysis of porcine DIO3 gene in two fetal stages and association analysis with carcass and meat quality traits
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猪DIO3基因在胎儿两期的印记分析及与胴体及肉质性状的关联分析
DOI：
10.1007/s11033-011-0983-z
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发表时间：
2011-06
影响因子：
2.8
通讯作者：
Deng, Chang-Yan
中科院分区：
文献类型：
--
作者：
Zhang, Peng-Peng;Li, Feng-E;Zheng, Rong;Deng, Chang-Yan
印记基因在哺乳动物的生长、发育和行为中发挥着重要作用。在本研究中，我们获得了猪Dio3(脱碘酶，碘甲腺原氨酸，III型)的1568bp的mRNA序列，并鉴定了其在猪胚胎发育过程中的印迹状态。完整的开放阅读框架(ORF)，编码278个氨基酸。猪Dio3mRNA主要在背脂中表达，在肝、子宫、肾、心脏、小肠、肌肉和胃中有少量表达，在脾和肺中几乎不表达。利用外显子A/C687单核苷酸多态技术，研究了不同品种猪的等位基因频率和猪胚胎组织中的印迹状态。结果表明，DiO3在所有受试组织中均有印迹。统计分析表明，Dio3基因多态性与瘦肉率(LMP)、脂肪率(FMP)、瘦肉比(RLF)、肩部脂肪厚度(SFT)、第6~7肋骨脂肪厚度(RFT)、臀部脂肪厚度(BFT)、眼肌面积(LEA)和肌内脂肪(IMF)等几乎所有脂肪沉积和屠体性状显著相关。
Imprinted genes play important roles in mammalian growth, development and behavior. In this study, we obtained 1568 bp mRNA sequence of porcine DIO3 (deiodinase, iodothyronine, type III), and also identified its imprinting status during porcine fetal development. The complete open reading frame (ORF) encoding 278 amino acids. The porcine DIO3 mRNA was expressed predominantly in backfat, mildly in liver, uterus, kidney, heart, small intestine, muscle and stomach, and almost absent in spleen and lung. A single nucleotide polymorphism in exon (A/C 687) was used to investigate the allele frequencies in different pig breeds and the imprinting status in porcine embryonic tissues. The results indicate that DIO3 was imprinted in all the tested tissues. Statistical analysis showed the DIO3 gene polymorphism was significantly associated with almost all the fat deposition and carcass traits, including lean meat percentage (LMP), fat meat percentage (FMP), ratio of lean to fat (RLF), shoulder fat thickness (SFT), sixth–seventh rib fat thickness (RFT), buttock fat thickness (BFT), loin eye area (LEA), and intramuscular fat (IMF).
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影响因子：
--
作者：
J. Köhrle
通讯作者：
J. Köhrle
影响因子：
4.8
作者：
Hernandez, Arturo;Garcia, Bibian;Obregon, Maria-Jesus
通讯作者：
Obregon, Maria-Jesus
影响因子：
4.4
作者：
A. Hernández;J. P. Park;G. Lyon;T. Mohandas;D. S. St. Germain
通讯作者：
A. Hernández;J. P. Park;G. Lyon;T. Mohandas;D. S. St. Germain
影响因子：
2.8
作者：
Z. Xu;H. Yang;Y. Xiong;C. Deng;Fenge Li;M. Lei;Bo Zuo
通讯作者：
Z. Xu;H. Yang;Y. Xiong;C. Deng;Fenge Li;M. Lei;Bo Zuo
影响因子：
4.8
作者：
G. Kuiper;W. Klootwijk;T. Visser
通讯作者：
G. Kuiper;W. Klootwijk;T. Visser