Spanish wastewater reveals the current spread of Monkeypox virus.
Spanish wastewater reveals the current spread of Monkeypox virus.
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DOI：
10.1016/j.watres.2023.119621
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发表时间：
2023-03-01
期刊：
影响因子：
12.8
通讯作者：
Sanchez, Gloria
中科院分区：
文献类型：
--
作者：
Giron-Guzman, Ines;Diaz-Reolid, Azahara;Truchado, Pilar;Carcereny, Albert;Garcia-Pedemonte, David;Hernaez, Bruno;Bosch, Albert;Pinto, Rosa Maria;Guix, Susana;Allende, Ana;Alcami, Antonio;Perez-Cataluna, Alba;Sanchez, Gloria
除鼻咽拭子外，还在唾液、精液、尿液和粪便样本等多种样本中检测到猴痘病毒（MPXV） DNA。利用西班牙以前开发的用于常规废水监测SARS-CoV-2 （VATar COVID-19）的环境监测网络，我们分析了西班牙不同地区废水中MPXV DNA的存在。在5月9日（2022年第19周）至8月4日（2022年第31周）期间，从24个不同的污水处理厂获得了样本（n = 312）。通过验证的铝吸附-沉淀法对病毒颗粒进行浓缩，qPCR程序使我们能够在2022年5月16日至8月4日收集的56份废水样品中检测MPXV DNA，其值范围为2.2 × 103至8.7 × 104基因组拷贝(gc)/L。本研究表明，通过qPCR可以在西班牙不同污水处理厂收集的纵向样本中重复性地检测到MPXV DNA。根据西班牙国家流行病学监测网络（RENAVE）的数据，截至2022年8月19日，西班牙共确诊6119例病例。然而，根据废水数据，报告的临床病例似乎被低估了，无症状感染可能比预期的更频繁。
Besides nasopharyngeal swabs, monkeypox virus (MPXV) DNA has been detected in a variety of samples such as saliva, semen, urine and fecal samples. Using the environmental surveillance network previously developed in Spain for the routine wastewater surveillance of SARS-CoV-2 (VATar COVID-19), we have analyzed the presence of MPXV DNA in wastewater from different areas of Spain. Samples (n = 312) from 24 different wastewater treatment plants were obtained between May 9 (week 19 of 2022) and August 4 (week 31 of 2022). Following concentration of viral particles by a validated aluminum adsorption-precipitation method, a qPCR procedure allowed us to detect MPXV DNA in 56 wastewater samples collected from May 16 to August 4, 2022, with values ranging between 2.2 × 103 to 8.7 × 104 genome copies (gc)/L. This study shows that MPXV DNA can be reproducibly detected by qPCR in longitudinal samples collected from different Spanish wastewater treatment plants. According to data from the National Epidemiological Surveillance Network (RENAVE) in Spain a total of 6,119 cases have been confirmed as of August 19, 2022. However, and based on the wastewater data, the reported clinical cases seem to be underestimated and asymptomatic infections may be more frequent than expected.
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影响因子：
9.8
作者：
de Jonge, Eline F.;Koelewijn, Jaap M.;van der Drift, Anne-Merel R.;Beek, Rudolf F. H. J. van der;Nagelkerke, Erwin;Lodder, Willemijn J.
通讯作者：
Lodder, Willemijn J.
DOI：
10.1016/j.scitotenv.2020.143870
发表时间：
2021-03-01
期刊：
The Science of the total environment
影响因子：
--
作者：
Pérez-Cataluña A;Cuevas-Ferrando E;Randazzo W;Falcó I;Allende A;Sánchez G
通讯作者：
Sánchez G
影响因子：
38.2
作者：
Hernaez, Bruno;Munoz-Gomez, Ana;Sanchiz, Africa;Orviz, Eva;Valls-Carbo, Adrian;Sagastagoitia, Inigo;Ayerdi, Oskar;Martin, Rocio;Puerta, Teresa;Vera, Mar;Cabello, Noemi;Vergas, Jorge;Prieto, Cristina;Pardo-Figuerez, Maria;Negredo, Anabel;Lagaron, Jose Maria;del Romero, Jorge;Estrada, Vicente;Alcami, Antonio
通讯作者：
Alcami, Antonio
影响因子：
158.5
作者：
Thornhill, John P.;Barkati, Sapha;Orkin, Chloe M.
通讯作者：
Orkin, Chloe M.
影响因子：
19
作者：
Inigo Martinez, Jesus;Gil Montalban, Elisa;Jimenez Bueno, Susana;Martin Martinez, Fernando;Nieto Julia, Alba;Sanchez Diaz, Jesus;Garcia Marin, Natividad;Cordoba Deorador, Esther;Nunziata Forte, Antonio;Alonso Garcia, Marcos;Humanes Navarro, Ana Maria;Montero Morales, Laura;Dominguez Rodriguez, Maria Jose;Carbajo Ariza, Manuel;Diaz Garcia, Luis Miguel;Mata Pariente, Nelva;Rumayor Zarzuelo, Mercedes;Velasco Rodriguez, Manuel Jose;Aragon Pena, Andres;Rodriguez Baena, Elena;Miguel Benito, Angel;Perez Meixeira, Ana;Ordobas Gavin, Maria;Lopaz Perez, Maria Angeles;Arce Arnaez, Araceli
通讯作者：
Arce Arnaez, Araceli