The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation.
The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation.
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DOI：
10.1083/jcb.200603042
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发表时间：
2006-09-11
期刊：
影响因子：
--
通讯作者：
Jayaram M
中科院分区：
文献类型：
--
作者：
Hajra S;Ghosh SK;Jayaram M
着丝粒蛋白A同源物Cse 4p是酿酒酵母中着丝粒组装和忠实染色体分离所必需的。它被认为是着丝粒染色质的精致标志。我们证明Cse 4位于2 μm质粒的分配位点STB。Cse 4p-STB的关联完全依赖于质粒分配蛋白Rep 1 p和Rep 2 p以及有丝分裂纺锤体的完整性。动粒突变ndc 10 -1将Cse 4p从着丝粒中排除，而不将其从STB中移出。在细胞周期中，Cse 4p-STB结合从G1/S持续到晚期末期。Cse 4p从STB染色质的释放可能通过纺锤体解体介导。功能性Cse 4p的缺乏破坏了RSC 2复合物对STB染色质的重塑，否定了STB处的Rep 2 p结合和粘着蛋白组装，并导致质粒错误分离。通过质粒偷猎特定的组蛋白变体，用着丝粒标签标记其分配位点，揭示了它在分离中实现染色体样保真度的另一种分子技巧。
The centromere protein A homologue Cse4p is required for kinetochore assembly and faithful chromosome segregation in Saccharomyces cerevisiae. It has been regarded as the exquisite hallmark of centromeric chromatin. We demonstrate that Cse4 resides at the partitioning locus STB of the 2-μm plasmid. Cse4p-STB association is absolutely dependent on the plasmid partitioning proteins Rep1p and Rep2p and the integrity of the mitotic spindle. The kinetochore mutation ndc10-1 excludes Cse4p from centromeres without dislodging it from STB. Cse4p-STB association lasts from G1/S through late telophase during the cell cycle. The release of Cse4p from STB chromatin is likely mediated through spindle disassembly. A lack of functional Cse4p disrupts the remodeling of STB chromatin by the RSC2 complex, negates Rep2p binding and cohesin assembly at STB, and causes plasmid missegregation. Poaching of a specific histone variant by the plasmid to mark its partitioning locus with a centromere tag reveals yet another one of the molecular trickeries it performs for achieving chromosome- like fidelity in segregation.
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影响因子：
16
作者：
Huang, J;Hsu, JM;Laurent, BC
通讯作者：
Laurent, BC
DOI：
10.1073/pnas.231212498
发表时间：
2001-10-23
影响因子：
11.1
作者：
Gordon, DM;Roof, DM
通讯作者：
Roof, DM
影响因子：
11.4
作者：
MURRAY, JAH;SCARPA, M;CESARENI, G
通讯作者：
CESARENI, G
影响因子：
11.4
作者：
Crotti, LB;Basrai, MA
通讯作者：
Basrai, MA
影响因子：
2
作者：
FUTCHER, AB
通讯作者：
FUTCHER, AB