Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea.

Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea.
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使用益生菌回收药渣及其对腹泻的治疗作用。

DOI:
10.1155/2017/4265898
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发表时间:
2017
影响因子:
4.6
通讯作者:
Xin H
Xin H
中科院分区:
医学3区
文献类型:
--
作者:
Meng F;Chen T;Ma D;Wang X;Zhao X;Tian P;Wang H;Hai Z;Shen L;Tang X;Wang X;Xin H

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药厂中药材加工过程中产生的残留物产生大量废物(药渣),其中主要包括环境污染和药用废物。为了解决这一问题,选择枯草芽孢杆菌(B.)、米曲霉(A.)和植物乳杆菌(L.)M3等益生菌来重复利用健胃消食片(JT)的药渣,并建立抗生素相关性腹泻(AAD)小鼠模型来评价药渣发酵的治疗效果。 上清液。结果表明,发酵上清液清除了77.8%的2,2-二苯基-1-三硝基苯肼(DPPH)、78%的O2•−、36.7%的•OH、39%的Fe2+螯合剂和716mg/L的还原力。鼠伤寒沙门氏菌 (S.)、肠炎沙门氏菌、福氏志贺氏菌 (Sh.)、大肠杆菌 (E.)、单核细胞增生李斯特菌 (L.)、Sh. 的抑制区。痢疾杆菌 301 和金黄色葡萄球菌 (S.) 分别为 17、14、19、18、20、19 和 20mm。体内结果表明,发酵上清液具有较高的腹泻抑制率(56%,p < 0.05),大大增强了抗生素对细菌多样性的破坏,恢复了约氏乳杆菌在治疗和恢复阶段的优势地位。因此,草药残留物和益生菌的组合表明探索这些材料的转化以开发 AAD 疗法的可能性。
Residues from herbal medicine processing in pharmaceutical plants create a large amount of waste (herb residues), which consists mainly of environmental pollution and medicinal waste. In order to resolve this problem, probiotics of Bacillus (B.) subtilis, Aspergillus (A.) oryzae, and Lactobacillus (L.) plantarum M3 are selected to reuse herb residue of Jianweixiaoshi tablets (JT), and an antibiotic-associated diarrhea (AAD) mouse model was established to evaluate the therapeutic effects of the herb residue fermentation supernatant. Our results indicated that the fermentation supernatant had scavenged 77.8% of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 78% of O2•−, 36.7% of •OH, 39% of Fe2+ chelation, and 716 mg/L reducing power. The inhibition zones for Salmonella (S.) typhimurium, S. enteritidis, Shigella (Sh.) flexneri, Escherichia (E.) coli, Listeria (L.) monocytogenes, Sh. dysenteriae 301, and Staphylococcus (S.) aureus were 17, 14, 19, 18, 20, 19, and 20 mm, respectively. The in vivo results indicated that the fermentation supernatant resulted in a high diarrhea inhibition rate (56%, p < 0.05), greatly enhanced the disruption of bacterial diversity caused by antibiotics, and restored the dominant position of L. johnsonii in the treatment and recovery stages. Therefore, the combination of the herb residue and probiotics suggests a potential to explore conversion of these materials for the possible development of therapies for AAD.
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