Expression of Src-like adapter protein mRNA is induced by all-trans retinoic acid.
Expression of Src-like adapter protein mRNA is induced by all-trans retinoic acid.
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Src 样接头蛋白 mRNA 的表达由全反式视黄酸诱导。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Y. Miura
中科院分区：
文献类型：
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作者：
T. Ohtsuki;K. Hatake;M. Ikeda;H. Tomizuka;Y. Terui;M. Uwai;Y. Miura
采用差异显示法，从atra依赖性分化的U937细胞的阶梯PCR产物中选择特异性条带，并与未处理的U937细胞进行比较。利用其中一种PCR产物筛选atra依赖性分化的U937细胞cDNA文库，克隆出src样适配蛋白（SLAP）。Northern blot分析显示，ATRA对U937细胞的SLAP mRNA有明显的诱导作用。环己亚胺的加入抑制了这种诱导作用，表明ATRA通过其他蛋白质的合成间接起作用。SLAP mRNA在HL60和NB-4中被诱导，而在K562和THP-1中未被诱导。有趣的是，这些由ATRA诱导的SLAP mRNA的细胞都表现出ATRA依赖性的细胞分化。SLAP与细胞分化的关系尚不清楚，但可能会转导细胞分化的信号。
By using a differential display method, specific bands were selected from ladder PCR products derived from ATRA-dependent differentiated U937 cells, in comparison with those of untreated U937. By screening the cDNA library of ATRA-dependent differentiated U937 cells with one of the PCR products, we cloned the src-like adapter protein (SLAP). Northern blot analysis of U937 cells with or without ATRA treatment indicated that the SLAP mRNA was clearly induced by ATRA. The induction was inhibited by the addition of cycloheximide, indicating that ATRA acted indirectly through synthesis of other proteins. The SLAP mRNA was induced in HL60 and NB-4 but not in K562 or THP-1. Interestingly, these cells in which SLAP mRNA was induced by ATRA all showed ATRA-dependent cell differentiation. The relationship between SLAP and cell differentiation is unclear, but SLAP may transduce a signal for cell differentiation.
影响因子：
56.9
作者：
REN, RB;MAYER, BJ;BALTIMORE, D
通讯作者：
BALTIMORE, D
小鼠 Src 样接头蛋白 (Slap) 基因及其假定的人类同源物 SLA 的染色体定位。
DOI：
10.1006/geno.1995.1289
发表时间：
1995
期刊：
Genomics.
影响因子：
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作者：
Angrist,M;Wells,DE;Chakravarti,A;Pandey,A
通讯作者：
Pandey,A