RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation.
RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation.
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DOI：
10.3389/fpls.2018.00259
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发表时间：
2018
影响因子：
5.6
通讯作者：
Pandey AK
中科院分区：
文献类型：
--
作者：
Aggarwal S;Kumar A;Bhati KK;Kaur G;Shukla V;Tiwari S;Pandey AK
提高微量营养素的生物利用度对于解决发展中国家的营养不良问题至关重要。用于解决微量营养素生物利用度的各种方法显示出有希望的迹象，特别是在谷类作物中。植酸（PA）被认为是一种主要的抗营养素，因为它能够螯合重要的微量营养素，从而限制它们的生物利用度。因此，通过调控PA的生物合成途径来克服不同作物间的多效性效应已成为研究热点。最近，我们报道了功能性小麦肌醇五磷酸激酶（TaIPK1）参与了PA的生物合成，但IPK1基因在小麦中的功能作用尚不清楚。在这项研究中，rnai介导的基因沉默对IPK1转录本进行了六倍体小麦。选取4个非分离性的小麦RNAi系（S3-D-6-1、S6-K-3-3、S6-K-6-10和S16-D-9-5）进行详细研究。TaIPK1转录物减少的T4种子纯合子转基因RNAi系显示PA降低28-56%。IPK1的沉默也导致成熟籽粒中游离磷酸盐的增加。虽然没有观察到穗的表型变化，但降低籽粒PA导致每穗种子数减少。随着籽粒PA的降低，铁（Fe）和锌（Zn）含量显著增加，从而提高了它们的摩尔比（Zn:PA和Fe:PA）。总的来说，这项工作表明IPK1是利用基因组编辑工具解决小麦籽粒矿物质积累的一个有希望的候选者。
Enhancement of micronutrient bioavailability is crucial to address the malnutrition in the developing countries. Various approaches employed to address the micronutrient bioavailability are showing promising signs, especially in cereal crops. Phytic acid (PA) is considered as a major antinutrient due to its ability to chelate important micronutrients and thereby restricting their bioavailability. Therefore, manipulating PA biosynthesis pathway has largely been explored to overcome the pleiotropic effect in different crop species. Recently, we reported that functional wheat inositol pentakisphosphate kinase (TaIPK1) is involved in PA biosynthesis, however, the functional roles of the IPK1 gene in wheat remains elusive. In this study, RNAi-mediated gene silencing was performed for IPK1 transcripts in hexaploid wheat. Four non-segregating RNAi lines of wheat were selected for detailed study (S3-D-6-1; S6-K-3-3; S6-K-6-10 and S16-D-9-5). Homozygous transgenic RNAi lines at T4 seeds with a decreased transcript of TaIPK1 showed 28–56% reduction of the PA. Silencing of IPK1 also resulted in increased free phosphate in mature grains. Although, no phenotypic changes in the spike was observed but, lowering of grain PA resulted in the reduced number of seeds per spikelet. The lowering of grain PA was also accompanied by a significant increase in iron (Fe) and zinc (Zn) content, thereby enhancing their molar ratios (Zn:PA and Fe:PA). Overall, this work suggests that IPK1 is a promising candidate for employing genome editing tools to address the mineral accumulation in wheat grains.
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影响因子：
3.7
作者：
Ali K;Warusevitane A;Lally F;Sim J;Sills S;Pountain S;Nevatte T;Allen M;Roffe C
通讯作者：
Roffe C
影响因子：
3.9
作者：
Rasmussen, Soren K.;Ingvardsen, Christina Ronn;Torp, Anna Maria
通讯作者：
Torp, Anna Maria
影响因子：
6.9
作者：
Gasparis, Sebastian;Orczyk, Waclaw;Nadolska-Orczyk, Anna
通讯作者：
Nadolska-Orczyk, Anna
DOI：
10.1186/1939-8433-6-12
发表时间：
2013-05-15
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Ali N;Paul S;Gayen D;Sarkar SN;Datta SK;Datta K
通讯作者：
Datta K
影响因子：
6.9
作者：
Bhati KK;Alok A;Kumar A;Kaur J;Tiwari S;Pandey AK
通讯作者：
Pandey AK