Electron microscope and biochemical observations of the surface active phospholipids on the articular surfaces and in the synovial fluid of the temporomandibular joint: a preliminary investigation.
Electron microscope and biochemical observations of the surface active phospholipids on the articular surfaces and in the synovial fluid of the temporomandibular joint: a preliminary investigation.
复制标题
颞下颌关节关节表面和滑液中表面活性磷脂的电子显微镜和生化观察：初步研究。
DOI：
--
复制
发表时间：
2001
影响因子：
1.9
通讯作者：
D. Nitzan
中科院分区：
文献类型：
--
作者：
E. Rahamim;H. Better;A. Dagan;D. Nitzan
目的
PURPOSE
The goal of this article is to investigate the surface-active phospholipids located on the articular surfaces and in the temporomandibular joint (TMJ) synovial fluid (SF) by means of electron microscopy and biochemical analysis.
MATERIALS AND METHODS
Synovial fluids and articular cartilage samples taken from 6 normally functioning TMJs were studied. The osmiophilic lining of human TMJ articular surfaces has been studied by using special nondestructive fixation procedures. To study the SF, negative staining technique has been used. In addition, thin-layer chromatography has been used to identify the phospholipids extracted from synovial fluid of human TMJs.
RESULTS
In the SF, granular bodies were identified with diameter of between 170 and 280 nm. Their diameter decreased dramatically when exposed to phospholipase-A(2). The amorphous and highly osmophilic material on the articular surface include membrane-bound vesicles (270 nm in diameter) with lamellated pattern surrounding the amorphous-dense core. Biochemical extraction revealed phosphatidylcholine as the major component of the polar lipids.
CONCLUSIONS
This preliminary study presents findings that suggest that phospholipids present in the TMJ may provide an efficient boundary lubrication that enables the disc to slide down the slope of the eminence on joint function.
细胞外磷脂酶A2。
影响因子：
--
作者：
Dennis,EA;Deems,RA;Yu,L
通讯作者：
Yu,L