Progesterone Receptor Membrane Component 1 Mediates Progesterone-Induced Suppression of Oocyte Meiotic Prophase I and Primordial Folliculogenesis.
Progesterone Receptor Membrane Component 1 Mediates Progesterone-Induced Suppression of Oocyte Meiotic Prophase I and Primordial Folliculogenesis.
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孕酮受体膜成分 1 介导孕酮诱导的卵母细胞减数分裂前期 I 和原始卵泡发生的抑制
DOI：
10.1038/srep36869
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发表时间：
2016-11-16
影响因子：
4.6
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Guo M;Zhang C;Wang Y;Feng L;Wang Z;Niu W;Du X;Tang W;Li Y;Wang C;Chen Z
Well-timed progression of primordial folliculogenesis is essential for mammalian female fertility. Progesterone (P4) inhibits primordial follicle formation under physiological conditions; however, P4 receptor that mediates this effect and its underlying mechanisms are unclear. In this study, we used anin vitroorgan culture system to show that progesterone receptor membrane component 1 (PGRMC1) mediated P4-induced inhibition of oocyte meiotic prophase I and primordial follicle formation. We found that membrane-impermeable BSA-conjugated P4 inhibited primordial follicle formation similar to that by P4. Interestingly, PGRMC1 and its partner serpine1 mRNA-binding protein 1 were highly expressed in oocytes in perinatal ovaries. Inhibition or RNA interference of PGRMC1 abolished the suppressive effect of P4 on follicle formation. Furthermore, P4-PGRMC1 interaction blocked oocyte meiotic progression and decreased intra-oocyte cyclic AMP (cAMP) levels in perinatal ovaries. cAMP analog dibutyryl cAMP reversed P4–PGRMC1 interaction-induced inhibition of meiotic progression and follicle formation. Thus, our results indicated that PGRMC1 mediated P4-induced suppression of oocyte meiotic progression and primordial folliculogenesis by decreasing intra-oocyte cAMP levels.
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DOI：
10.1073/pnas.1207854109
发表时间：
2012-10-23
影响因子：
11.1
作者：
Hunt, Patricia A.;Lawson, Crystal;VandeVoort, Catherine A.
通讯作者：
VandeVoort, Catherine A.
影响因子：
4.8
作者：
Kezele, P;Skinner, MK
通讯作者：
Skinner, MK
影响因子：
4.1
作者：
Nilsson, Eric E.;Skinner, Michael K.
通讯作者：
Skinner, Michael K.
影响因子：
3.5
作者：
Mansouri, Mahmoud Reza;Schuster, Jens;Dahl, Niklas
通讯作者：
Dahl, Niklas
影响因子：
3.8
作者：
Nilsson, Eric E.;Stanfield, Jacob;Skinner, Michael K.
通讯作者：
Skinner, Michael K.