Silk fibroin scaffolds promote formation of the ex vivo niche for salivary gland epithelial cell growth, matrix formation, and retention of differentiated function.
Silk fibroin scaffolds promote formation of the ex vivo niche for salivary gland epithelial cell growth, matrix formation, and retention of differentiated function.
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丝素蛋白支架促进唾液腺上皮细胞生长、基质形成和分化功能保留的离体生态位的形成。
DOI：
10.1089/ten.tea.2014.0411
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发表时间：
2015
影响因子：
--
通讯作者：
Yeh,Chih-Ko
中科院分区：
文献类型：
--
作者：
Zhang,Bin-Xian;Zhang,Zhi-Liang;Lin,AlanL;Wang,Hanzhou;Pilia,Marcello;Ong,JooL;Dean,DavidD;Chen,Xiao-Dong;Yeh,Chih-Ko
唾液腺功能低下通常是由多种原因引起的，包括使用各种药物、头部和颈部肿瘤的放射治疗、自身免疫性疾病、糖尿病和衰老。由于缺乏针对这种疾病的治疗方法，而且成人唾液腺几乎没有再生能力，因此需要基于细胞的治疗来恢复唾液腺功能。这些治疗策略的发展需要建立一种能够复制唾液腺细胞“利基”的系统，以支持唾液腺前体细胞的增殖和分化。本研究建立了三维丝素蛋白支架(SFS)与大鼠颌下腺(SM)和腮腺(PG)原代唾液腺上皮细胞(PSGECs)的培养体系，并对其进行了鉴定。在SFS上生长的pSGECs，而不是在组织培养塑料上生长的pSGECs，形成具有类似分泌腺泡的形态特征的细胞聚集体。在SFS上长时间培养后，两种类型的细胞都将高水平的淀粉酶释放到培养基中。值得注意的是，在SFs上培养的PG来源的细胞，而不是SM细胞，对β肾上腺素能受体激动剂异丙肾上腺素有反应，酶的释放增加。这种行为模仿体内唾液腺的行为。PSGECs在SFS上培养形成的脱细胞细胞外基质(ECM)含有基底膜的主要成分IV型胶原。这些结果表明，生长在SFS而不是TCP上的pSGECs保留了分化唾液腺的重要功能和结构特征，并产生了一种模仿天然唾液腺细胞生态位的ECM。这些结果表明，SFS有可能作为一种支架在体外建立唾液腺细胞巢蛋白，并可能提供一种诱导多潜能干细胞为患者再生这些组织提供具有治疗意义的数量的唾液腺前体细胞的方法。
Salivary gland hypofunction often results from a number of causes, including the use of various medications, radiation for head and neck tumors, autoimmune diseases, diabetes, and aging. Since treatments for this condition are lacking and adult salivary glands have little regenerative capacity, there is a need for cell-based therapies to restore salivary gland function. Development of these treatment strategies requires the establishment of a system that is capable of replicating the salivary gland cell “niche” to support the proliferation and differentiation of salivary gland progenitor cells. In this study, a culture system using three-dimensional silk fibroin scaffolds (SFS) and primary salivary gland epithelial cells (pSGECs) from rat submandibular (SM) gland and parotid gland (PG) was established and characterized. pSGECs grown on SFS, but not tissue culture plastic (TCP), formed aggregates of cells with morphological features resembling secretory acini. High levels of amylase were released into the media by both cell types after extended periods in culture on SFS. Remarkably, cultures of PG-derived cells on SFS, but not SM cells, responded to isoproterenol, a β-adrenergic receptor agonist, with increased enzyme release. This behavior mimics that of the salivary glandsin vivo. Decellularized extracellular matrix (ECM) formed by pSGECs in culture on SFS contained type IV collagen, a major component of the basement membrane. These results demonstrate that pSGECs grown on SFS, but not TCP, retain important functional and structural features of differentiated salivary glands and produce an ECM that mimics the native salivary gland cell niche. These results demonstrate that SFS has potential as a scaffold for creating the salivary gland cell nichein vitroand may provide an approach for inducing multipotent stem cells to provide therapeutically meaningful numbers of salivary gland progenitor cells for regenerating these tissues in patients.
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影响因子：
2.2
作者：
D'Avola, Tsunae Elizabeth;Ogawa, Koichi;Watanabe, li-sei
通讯作者：
Watanabe, li-sei
影响因子：
--
作者：
Wei, Cindy;Larsen, Melinda;Yamada, Kenneth M.
通讯作者：
Yamada, Kenneth M.
影响因子：
4
作者：
Lai, Yanlai;Sun, Yun;Chen, Xiao-Dong
通讯作者：
Chen, Xiao-Dong
食管机械和化学刺激对健康个体唾液粘蛋白分泌的影响
DOI：
10.1097/00000441-199407000-00006
发表时间：
1994
期刊：
The American Journal of the Medical Sciences
影响因子：
--
作者：
J. Sarosiek;R. Rourk;R. Piascik;D. Hetzel;R. McCallum
通讯作者：
R. McCallum
影响因子：
6.4
作者：
Aframian, Doron J.;Palmon, Aaron
通讯作者：
Palmon, Aaron